国际神经病学神经外科学杂志2012，Vol.39Issue(5)：395-399,5.
90K/Mac-2 BP在人脑星形细胞瘤中的表达
Expression of 90K/ Mac-2 BP in human brain astrocytic tumors
陈鑫 1刘运生 1刘志雄 1杨魁 1李春涛 1曾瑜 1龚璇 1陈敏1
作者信息
- 1. 中南大学湘雅医院神经外科,湖南长沙410008
- 折叠
摘要
Abstract
Objective To investigate the expression of 90K mRNA and protein in human brain astrocytomas. Methods The expression of 90K mRNA in human normal brain tissue and astrocytoma tissue was detected by RT-PCR; The expression of 90K protein in human normal brain tissue and astrocytoma tissue was detected by WB. Results RT-PCR reveal that 90K mRNA was lowly expressed in normal human brain tissues(0. 116 ±0.017). The 90K mRNA expression was significantly higher in astrocytomas (0. 407 ±0. 151 ) than that in normal human brain tissues (t = 6. 065, P < 0. 05) , and significantly higher in high grade astrocytomas ( WHO III-IV) (0.516 ±0. 128) than in low grade(WH0 I-II) (0.295 ±0.067) (t =8. 138, P<0.05 ). WB reveal that 90 K protein was lowly expressed in normal human brain tissues (0. 291 ± 0. 064). The 90K protein expression was significantly higher in astrocytomas ( 1. 163 ± 0. 391) than that in normal human brain tissues (t = 15. 68 , P < 0. 05) , and significantly higher in high grade astrocytomas (1.415 ±0.318) than in low grade (0.902 ±0.272 ) (t =6. 539, P<0.05 ). The expression of 90K mRNA was in accordance with the expression of 90K protein both in astrocytomas and normal human brain tissues. Conclusions 90K expressed positive in astrocytomas. The expression of 90K is higher in higher grade astrocytomas. 90K may contribute to occurrence and progression of astrocytomas. 90K protein may be a astrocytomas-associated antigen and target antigen in immunotherapy.关键词
90K/ Mac-2 BP/星形细胞瘤/RT-PCR/Western blotKey words
90K/ Mac-2 BP/ astrocytomas/ RT-PCR/ Western blot引用本文复制引用
陈鑫,刘运生,刘志雄,杨魁,李春涛,曾瑜,龚璇,陈敏..90K/Mac-2 BP在人脑星形细胞瘤中的表达[J].国际神经病学神经外科学杂志,2012,39(5):395-399,5.
