南方农业学报2012，Vol.43Issue(9)：1257-1261,5.DOI:10.3969/j:issn.2095-1191.2012.09.1257
EPSPS基因克隆及其表达载体的构建
Cloning of EPSPS gene and construction of its expression vector
摘要
Abstract
[Objective]This research aimed to clone EPSPS gene of soybean and construct its expression vector in order to provide the theoretical basis and the technical reservation for the cultivation of glyphosate-resistant crop.[Method]The total genome of glyphosate-resistant soybean was used as templates.The EPSPS gene was amplified,and its plant expression vector,PCAMBIA1300-UBI-GFP-EPSPS,was constructed; afterwards,it was translated into Agrobacterium tumefaciens in order to carry out the crop's genetic transformation.[Result]The full-length of the amplified EPSPS gene was 1368 bp,which encoded 455 amino acids.The sequencing result showed the identical CDS sequence as the known one in GenBank (AF464188.1).The plant expression vector of EPSPS was constructed successfully and translated into Agrobacterium tumefaciens EHA105.[Conclusion]After its genetic transformation,this expression vector could be used for glyphosate-resistant trait improvements in sweet sorghum and other monocotyledon crops.关键词
大豆/EPSPS基因/抗草甘膦/表达载体/构建Key words
soybean/ EPSPS gene/ glyphosate resistance/ expression vector/ construction分类
农业科技引用本文复制引用
谢鸿锴,禤维言,王磊,冯斗..EPSPS基因克隆及其表达载体的构建[J].南方农业学报,2012,43(9):1257-1261,5.基金项目
Special project for cooperation between Nanning City and Guangxi University(200901029B) （200901029B）
and project Initiating Foundation for Doctoral Degree Holders of Guangxi University (X041054) （X041054）
