南方农业学报2012，Vol.43Issue(9)：1269-1272,4.DOI:10.3969/j:issn.2095-1191.2012.09.1269
一株高产脂肪酶产生菌16S rDNA的序列分析
Sequence analysis of 16S rDNA derived from a high yield lipase strain
摘要
Abstract
[Objective]The present study was conducted to identify the separated high yield lipase strain to provide theoretical research references and to enhance the high yield lipase strain's transformation and application. [Method]A strain of bacterium with high yield lipase JLIT-4 was isolated from the sewage of a canteen, then its genomic DNA was extracted. The gene fragments of 16S rDNA were amplified using 16S rDNA universal primers and connected to pUC19-T vector; the fragments are then transformed into E. Coli DH5X. The positive clones identified by the PCR method were cultured and sequenced. [Result]Quality genome DNA was successfully extracted. The 16S rDNA gene fragments of newly isolated strain were amplified with the length of 1528 bp. According to comparison analysis of BLAST, 16S rDNA sequence similarity between the strains and Burkholderia(DQ355168) were 97%, so the lipase producing strains were identified as gram-negative bacteria that were most similar to the structures of Burkholderia. [ Conclusion ]The high-yield lipase JLΠ-4 was primarily identified as Burkholderia gladioli.关键词
脂肪酶/细菌/16S rDNA/序列分析Key words
lipase/ bacteria/ 16S rDNA/ sequence analysis分类
化学化工引用本文复制引用
孙新城,马淑玲,张玲丽,张浩,景建洲..一株高产脂肪酶产生菌16S rDNA的序列分析[J].南方农业学报,2012,43(9):1269-1272,4.基金项目
河南省重点科技攻关计划项目(102102310093) （102102310093）
