南方农业学报2012，Vol.43Issue(9)：1349-1352,4.DOI:10.3969/j:issn.2095-1191.2012.09.1349
广西主栽中高温平菇耐硒能力比较及生物富硒条件初步优化
Comparison on Se tolerance of high temperature oyster mushroom varieties in Guangxi and their Se-enriching condition optimization
摘要
Abstract
[ObjectivejThe present research was conducted to screen out high Se resistant oyster mushroom varieties and preliminarily optimize Se-accumulation condition of oyster mushroom mycelia in order to provide scientific references for developing efficient, safe, and high Se tolerant and edible mushroom products. [Method]Colony diameter, mycelia growth vigor of 13 high temperature oyster mushroom strains in solid medium with different ratios of Na2SeO3, were compared to select Se tolerant oyster mushroom varieties combined with submerged fermentation. The Na2SeO3 concentration, initial pH, inoculum quantity, and medium volume for high Se-tolerant oyster mushroom varieties were optimized using single factors test. [Result]With the lowest tolerance coefficient of 0.4, Jiangdu 302 displayed the strongest Se tolerance compared to that of the other 12 oyster mushroom varieties. It was found that the optimal conditions for Jiangdu 302 strain, culturing on shaking table with rotation rate of 160 r/min at 26℃ for 7 days, were as follows: Na2SeO3 concentration 5-15 mg/L, initial pH 5.0-7.0, inoculum quantity 5-15%, and medium volume 80-120 mL in the single factor experiment. [Conclusion]Obvious differences were found in Na2SeO3 tolerance of all 13 strains, yet Jiangdu 302 showed the strongest Se tolerance ability. Na2SeO3 concentration, initial pH value, inoculum quantity, and medium volume influenced the Se-enrichment of oyster mushroom mycelia tremendously. Amongst the experimental combinations, Se content in Jiangdu 302 was the highest under Na2SeO3 concentration 10mg/L,initial pH value 6.0, inoculum 10%, and medium volume 100 mL/250 mL.关键词
中高温平菇/耐硒/富硒/条件优化/广西Key words
high temperature oyster mushroom/ Se tolerance/ Se accumulation/ condition optimization/ Guangxi分类
农业科技引用本文复制引用
覃晓娟,熊建文,何忠,王灿琴,韦仕岩,高忠奎..广西主栽中高温平菇耐硒能力比较及生物富硒条件初步优化[J].南方农业学报,2012,43(9):1349-1352,4.基金项目
广西农业科学院基本科研业务专项项目(桂农科2011YM17) （桂农科2011YM17）
