南方农业学报2012，Vol.43Issue(9)：1391-1394,4.DOI:10.3969/j:issn.2095-1191.2012.09.1391
蛇含委陵菜提取物对人工感染鸡白痢的治疗效果
Therapeutic effect of Potentilla kleiniana extract against experimental Salmonella pullorum in chicken
摘要
Abstract
[Objective]The present experiment was aimed to evaluate the therapeutic effect of Potentilla kleiniana extract against experimental Salmonella pullorum in chicken. [Method]Five extracting methods viz., water decoction, water decoction combined with ethanol extracting, microwave extracting, ethyl acetate extracting and ethanol grading extracting were used to extract Potentilla kleiniana and minimal inhibitory concentration (MIC) in several extracts of different polarity zones were measured to screen out the best extraction method; subsequently, the Potentilla kleiniana extract with three different dosages (high, moderate, and low) were drenched to treat one-day-old chicks infected with artificial Salmonella pullorum, then the therapeutic effects were assessed using norfloxacin as the medical control. [Result]The water extract, obtained from water decoction, showed the most potent antimicrobial activity to Salmonella pullorum and Escherichia coli in chicken, and the MIC of Salmonella pullorum and Escherichia coli were 5.21 and 10.41 mg/mL, respectively. The optimal treatment effective rate of chicken reached 86.7% through drenching 0.75 mL/chicken once a day for 3 days consecutively after infecting Salmonella pullorum for 18 hours. [Conclusion]Safe, inexpensive, and low-toxic Potentilla kleiniana extract could effectively inhibit gram-negative bacterium; hence, Potentilla kleiniana extract is of high research and developmental value.关键词
蛇含委陵菜/提取物/鸡白痢/沙门氏菌/治疗效果Key words
Potentilla kleiniana/ extract/ pullorum disease/ Salmonella pullorum/ therapeutic effect分类
农业科技引用本文复制引用
陶俊宇,胡庭俊,班丽萍..蛇含委陵菜提取物对人工感染鸡白痢的治疗效果[J].南方农业学报,2012,43(9):1391-1394,4.基金项目
广西自然科学基金重点项目(2010GXNSFD013022) （2010GXNSFD013022）
广西研究生教育创新计划资助项目(GXUIIT32526) （GXUIIT32526）
