南方农业学报2012，Vol.43Issue(11)：1626-1632,7.DOI:10.3969/j:issn.2095-1191.2012.11.1626
DHAR与草莓AsA积累关系及DHAR RNAi遗传转化研究
Relationship between DHAR and AsA accumulation in strawberry and its DHAR RNAi genetic transformation
摘要
Abstract
This research preliminarily identified strawberry DHAR enzyme activity and its relationship with AsA and studied the effects of DHAR on regulating AsA accumulation using RNAi technologies in order to provide theoretical basis for breeding high AsA strawberry varieties. [ Method ]The AsA and DHAR activities of strawberry fruit during different periods were measured and correlatively analyzed. By the Agrohacterium tumefaciens transformation mediated method, the RNAi plant expression vector of DHAR gene were constructed and introduced into strawberry. [Result] The AsA content of strawberry fruit during ripening stage displayed an increasing trend, in which during the late growth period, the AsA content showed the largest increase. As the strawberry fruits matured, the DHAR activities increased gradually and measured the highest during the late growth stage. The plant expression vector RNAi (Part27-IDHAR) of the DHAR gene was constructed, and the Agrobacterium tumefaciens transformation mediated method was used to trnasfer the gene into strawberry tissues. As a result, a total of 7 PCR-positive plants were obtained, and a preliminary description of the target gene was integrated into the strawberry tissues. [Conclusion]DHAR activity was significantly positive correlated to AsA accumulation. The obtained transformation plants could be as material for further study.关键词
草莓/DHAR/AsA/RNAi/遗传转化Key words
strawberry/ DHAR/ AsA/ RNAi/ genetic transformation分类
农业科技引用本文复制引用
张丙秀,李柱刚,高媛,刘丹,高庆玉..DHAR与草莓AsA积累关系及DHAR RNAi遗传转化研究[J].南方农业学报,2012,43(11):1626-1632,7.基金项目
公益性行业体系专项项目(201103037) （201103037）
东北农业大学博士启动基金项目 （）
