南方农业学报2012，Vol.43Issue(11)：1642-1645,4.DOI:10.3969/j:issn.2095-1191.2012.11.1642
利用AFLP鉴定沙田柚新种质
Identification of new Shatian pumelo (Citrus grandis L.) germplasm by AFLP
摘要
Abstract
The genetic diversities of Shatian pummelo (Citrus grandis L.) were identified and classified in order to provide references for future usage. [ Method ]Out of the 64 selected pairs of AFLP primer combination, 12 pum-melos materials were carefully classified and analyzed. [Result]From the 64 primers, 12 pairs of AFLP primer combinations were screened out. The 12 tested materials were amplified with 399 clear bands, in which each pair of primers amplified 25 to 49 bands; the average number of amplified bands for each pair of primers was 33.25. The polymorphic band number was 91 , and the polymorphism ratio was 22.8%. The similarity coefficients of the tested materials were between 0.752 and 0.956. The AFLP identification results showed that similarity coefficient of Guiyou 1 with Shatian pomelo was 0.956, and the similarity coefficients of early-ripening Shatian pomelo and Shatian pomelo was 0.920. The two pomelo varieties, early梤ipening sour pomelo and Fujian sour pomelo, were distantly related to other pomelo varieties. Using the similarity coefficient threshold of 0.83, the 12 tested pomelo materials were divided into 5 groups. [Conclusion]The AFLP molecular marker technology could successfully separate the 12 tested pomelo materials. Early-ripening Shatian pomelo and Guiyou 1 were different from the other pomelo variations, and could be directly exploited as variety resources.关键词
沙田柚/桂柚1号/早熟沙田柚/遗传鉴定/AFLPKey words
Shatian pomelo/ Guiyou 1/ early-ripening Shatian pomelo/ genetic identification/ AFLP分类
农业科技引用本文复制引用
肖远辉,麦适秋,傅翠娜,区善汉,梅正敏,廖奎富,徐粹明..利用AFLP鉴定沙田柚新种质[J].南方农业学报,2012,43(11):1642-1645,4.基金项目
国家科技部农业科技成果转化项目(2011GB2E100006) （2011GB2E100006）
广西科学研究与技术开发项目(桂科攻1123003-3A,桂科转1123013-7) （桂科攻1123003-3A,桂科转1123013-7）
广西科学基金项目(2010GXNSFA013077) （2010GXNSFA013077）
