南方农业学报2012，Vol.43Issue(11)：1672-1675,4.DOI:10.3969/j:issn.2095-1191.2012.11.1672
应用正交试验设计优选番木瓜组培苗生根培养基研究
Rooting medium optimization of Carica papaya plantlets by using orthogonal design
摘要
Abstract
This research studied the different compositional effects of rooting medium on rooting induction of Carica papaya plantlets in order to provide references for further tissue culture and rapid propagation. [Method]Using the eighth generation tissue culture plantlets' lateral buds of the Carica papaya Tainong No.l as test materials in this experiment, the plantlets were inoculated to the correspondent matching culture medium; different concentrations of IBA, NAA, and AC were applied as the rooting medium for rooting culture, and the best rooting medium were selected by using the orthogonal experiment design L9(34). [Result]In the MS, 1/2MS, and 3/2MS culture medium, the rooting rate of plantlets showed significant difference, and the induction effects were ranked in the following order: MS>1/2MS>3/2MS. Different rooting rates of papaya with various IBA concentrations were ranked in the following order: 2.0 mg/L>2.5 mg/L> 1.0 mg/L IBA and the optimal IBA concentration ranged between 2.0-2.5 mg/L. Different NAA concentrations on the rooting induction of papaya were ranked in the following order: 0.05 mg/L >0.10 mg/L>0.00 mg/L, and the optimal concentration was 0.05 mg/L. The activated carbon effects on the rooting induction of papaya were ranked in the following order: 0.002%>0.005%>0.000%, and the optimal concentration was 0.002%. Lastly, the MS culture medium, IBA, NAA, and AC on the papaya rooting rate were ranked in the following order: IBA>AC>NAA>MS culture medium. [Conclusion] The optimal medium combination for papaya rooting induction was MS+2.0 mg/L IBA+0.05 mg/L NAA+0.002% AC.关键词
番木瓜/组培苗/生根培养基/正交试验/激素配比/优选Key words
Carica papaya/ plantlet/ rooting medium/ orthogonal design分类
农业科技引用本文复制引用
唐文忠,王小媚,黄伟雄,廖芬,黄茂康..应用正交试验设计优选番木瓜组培苗生根培养基研究[J].南方农业学报,2012,43(11):1672-1675,4.基金项目
广西自然科学基金项目(桂科自0640050) （桂科自0640050）
