南方农业学报2012，Vol.43Issue(11)：1692-1696,5.DOI:10.3969/j:issn.2095-1191.2012.11.1692
核桃根、茎、叶提取物对蚕豆的遗传毒性分析
Genetic toxicity of active ingredients extracted from roots,stems, and leaves of walnut on Vicia faba
摘要
Abstract
In order to provide scientific evidences for the development of pollution-free botanical herbicides, this study explored the allelopathic mechanism of different extracts from the roots, stems, and leaves of walnut (Juglans regia L.). [ Method jThe Vicia (aba root growth test and micronucleus test were used to study the effects of the different ingredients derived from the roots, stems, and leaves of walnut on Vicia faba growth and their genetic toxicity. [ResultJThe research results showed that the Vicia faba root grew slowly after being cultured by the n-butanol extracts (0.025, 0.050, 0.100 g/mL) from walnut roots and leaves for 24 and 48 hrs, respectively. When the extracts concentration was greater than 0.050 g/mL, the Vicia faba root, which was cultured for 72 hrs, stopped growing. The micronuclear test indicated that the n-butanol extracts of roots and leaves could inhibit the growth of Vicia faba root tip cells and damage the genetic material, which resulted in the mitotic index of Vicia faba root tips to decrease and micronuclear rate to increase. The experimental group's research results had significant difference compared with the controlled group's research results (P<0.05) and the concentration and time effects in the experimental concentration range (0.025-0.100 g/mL) was significant. [Conclusion]The walnut roots and leaves had allelochemicals genetic toxicity, which inhibited the division of plant cells and damaged the genetic materials of cells; this resulted in the irreversible genetic damage to the nearby plants.关键词
核桃/化感作用/有丝分裂/遗传毒性Key words
Juglans regia L./ allelopathy/ mitotic / genetic toxicity分类
农业科技引用本文复制引用
黄元河,潘乔丹..核桃根、茎、叶提取物对蚕豆的遗传毒性分析[J].南方农业学报,2012,43(11):1692-1696,5.基金项目
广西教育厅科研项目(201106LX428) （201106LX428）
右江民族医学院项目(右医科字[2011]1号201011) （右医科字[2011]1号201011）
