南方农业学报2012，Vol.43Issue(11)：1758-1764,7.DOI:10.3969/j:issn.2095-1191.2012.11.1758
基于12S rRNA和16S rRNA序列的龟鳖类系统进化特征研究
Phylogenetic characteristics of turtles based on 12S rRNA and 16S rRNA sequence
摘要
Abstract
The experiment was conducted to analyze the phylogenetie relationships of terrapin in order to offer basic theoretical basis on terrapin resources protection and development. [Method]12S rRNA and 16S rRNA sequences of Chelydra serpentina were obtained by using PCR amplification and sequencing. The homologous sequences of the other terrapins were downloaded from NCBI and analyzed together with the sequences of Chelydra serpentina. Inter-specific, inter-genus , and inter-family genetic distances were calculated based on the Kimura-2-parameter model, and the phylogenetie trees were constructed using the methods of neighbor joining (NJ) , maximum parsimony (MP), and maximum likelihood (ML). [Result]After alignment, 394 bp consensus sequences of 12S rRNA and 544 bp consensus sequences of 16S rRNA were obtained, which merged together and became the 938 bp combined sequence. In the combined sequence, 327 variable sites were detected, accounting for 34.9% of total sequences. Furthermore, there were 222 parsimony informative sites and 105 singleton sites. The average content of T, C, A, and G were 23.6, 24.1 , 33.5, and 18.7%, respectively. The content of A+T (57.1%) is greater than that of G+C (42.8%), and the ratio between transition and transversion was 2.53. The kimura-2-parameter distances between Mauremys ranged from 0.021 to 0.060, with an average measured at 0.467; the distances among 8 genus within Bataguridae ranged from 0.022 to 0.110, with an average measured at 0.0724; lastly, the distances among 7 families within Testudines (excluding Platysternon) were from 0.071 to 0.123, with an average value measured at 0.105. Phylogenetie tree analysis results suggested that Rhinoclemmys and Testudinidae should be clustered into one cataloge, then followed by Bataguridae. Ocadia sinensis and Chinemys megalocephala should be clustered into Mauremys all together. Chelydridae, Cheloniidae, and Dermochelyidae should be clustered into a single categary. Kinosternidae should be clustered into a category alone. [ Conclusion ] Chinemys megalocephala and Ocadia sinensis should be incorporated into Mauremys, and Platystemon megacephalum could be separated from Chelydridae and vested in Platystemidae independently.关键词
龟鳖类/12S rRNA/16S rRNA/Kimura双参数遗传距离/系统进化树Key words
turtles/ 12SrRNA/ 16S rRNA/ Kimura-2-parameter distance/ phylogenic tree分类
农业科技引用本文复制引用
刘海情,刘楚吾,刘丽..基于12S rRNA和16S rRNA序列的龟鳖类系统进化特征研究[J].南方农业学报,2012,43(11):1758-1764,7.基金项目
广东省科技计划项目(2010B020308004) （2010B020308004）
