广西医科大学学报2012，Vol.29Issue(5)：664-668,5.
TrkB、P75NTR在颞叶癫痫大鼠海马中的表达及托吡酯干预的研究
EXPRESSIONOF TRKB AND P75NTR IN HIPPOCAMPUS OF RAT MODEL OF TEMPORAL LOBE EPILEPSY AND THE EFFECT OF TOPIRAMATE
摘要
Abstract
To investigate the characteristics of the histological changes and the brain derived neu-rotrophic factor (BDNF) receptor TrkB and P75NTR expression in hippocampus of a temporal lobe epilepsy (TLE) rat model induced by kainic acid (KA) after topiramate (TPM) intervention; and explore the effect of TrkB and P75NTR in TLE and the antiepileptic mechanism of topiramate. Methods: Adult rats were made to be a model of TLE by KA inducing status epilepticus. Behavioral changes were observed. TrkB and P75NTR protein was studied by immunohistochemistry at time points of Id, lw, 2w, 3w and 4w. Hipp-ocampal neuron loss was detected by Nissl's staining. Results: (1) epileptic seizures were decreased after TMP intervention. (2) After KA-induced SE, cell loss in CA1 pyramidal cells and hillar neurons was prominent, achieving peak in 4w. The impairment of hippocampal neurons lessened significantly after TMP intervention. (3) after KA-induced SE, TrkB expression was increased significantly on PoDG and CA3 re-gions( P <0. 01)in ld-4w and lasted for 4 weeks; but was increased only in Id on CA1 region ( P <0. 01) ; P75NTR expression was increased on PoDG, CA1 and CA3 regions ( P <0. 01) and lasted for 2 weeks on PoDG and CA3 regions ( P <C0. 05). The expression of TrkB and P75NTR was increased significantly on all of mention above regions either the time or the space after TPM intervention. Conclusion: The expression increased and lasting long time in TrkB and P75NTR in hippocampus were acted through their receptors and perhaps contributed to the persistent structure and functional changes of the formation in epilepsy. TPM intervention can obviously lessen the level of the KA-induced seizure and have an obvious protection to hippocampal neuron injury. After KA-induced SE, TPM probably has a potential protective effect during sei- zures by down-regulating Trk and P75NTR expression and then decreasing hippocampal neuron injury.关键词
颞叶癫痫/BDNF/TrkB/P75NTR/托吡酯Key words
temporal lobe epilepsy/ BDNF/ TrkB/ P75NTR/ topiramate分类
医药卫生引用本文复制引用
余璐,梁秀琳,郑金瓯..TrkB、P75NTR在颞叶癫痫大鼠海马中的表达及托吡酯干预的研究[J].广西医科大学学报,2012,29(5):664-668,5.基金项目
广西科学基金资助项目(No.桂科自0640118) （No.桂科自0640118）
广西卫生厅自筹课题(No.桂卫Z2010363) （No.桂卫Z2010363）
