广西医科大学学报2012，Vol.29Issue(5)：669-672,4.
广西莪术中提取姜黄素及联合顺铂诱导人卵巢癌细胞凋亡的研究
EFFECTS OFCURCUMIN EXTRACT COMBINED WITH CISPLATIN ON APOPTOSIS OF SKOV3 CELLS
摘要
Abstract
To improve the curcumin content extracted from Curcuma kwangsiensis with surfactant and study its effect on the apoptosis of human ovarian carcinoma SKOV3 combined with cisplatin. Methods: Moistened with appropriate quantity of surfactant, the curcumin was extracted by alcohol and assayed by HPLC for its ratio. The cells inoculated in the logarithm phase were administrated with 25, 50, and 100 μmol/L of curcumin or combined medication; 25, 50, and 100 μmol/L of curcumin was combined with 10 μmol/L of cisplatin, respectively, cultured for 48h and their absorbances was assayed by MTT method to calculate the inhibition rate of the cell growth. The normal controls, the 50 jumol/L of curcumin group, the 10 jumol/L of cisplatin group, and the 50 jumol/L of curcumin combined with 10 jumol/L of cisplatin was AO/EB stained to observe their apoptosis. Results: A maximum yield was obtained from each 10 g of Curcuma bulk material moistened by 10 mL 1 % of Sodium dodecyl sulfate (SDS) for 30 min, extracted with double refluxed ethanol for 1. 5 h per time. The MTT outcome and observation of Fluorescent staining indicated a synergistic induction to SKOV3 apoptosis by curcumin and cisplatin combination. Conclusion: SDS affects significantly to the curcumin extraction from curcuma. SKOV3 apoptosis can be synergistically inducted by curcumin in Curcuma kwangsiensis and cisplatin combination.关键词
莪术/姜黄素/表面活性剂/人卵巢癌细胞Key words
Curcuma/ curcumin/ surfactant/ ovarian cancer分类
医药卫生引用本文复制引用
蒋兴明,黄兴振,苏延旭,宋慧,黄静,徐星新..广西莪术中提取姜黄素及联合顺铂诱导人卵巢癌细胞凋亡的研究[J].广西医科大学学报,2012,29(5):669-672,4.基金项目
广西2011年研究生教育创新计划资助项目(No.2011105981007M174) （No.2011105981007M174）
