工业微生物2012，Vol.42Issue(6)：8-13,6.DOI:10.3969/j.issn.1001-6678.2012.06.002
卧式生物反应器固定化少根根霉发酵生产脂肪酶
Production of lipase by immobilized cells from Rhizopus arrhizus in a horizontal bioreactor
摘要
Abstract
Production of lipase by immobilized cells from Rhizopus arrhizus was studied in a horizontal bioreactor with polyurethane foam as a porous biomass support. The effects of immobilized areas, aeration rate, rotating rate and volume of broth on the lipase activity were investigated. The optimum fermentation conditions obtained were as follows; aeration rate 5 L/min, rotation speed 40 r/min, and 3. 5 L medium with the cylindrical bracket in 6 L horizontal bioreactor. The highest lipase activity of 95 U/mL and the lipase productivity of 2021 U/(h · L) were achieved in batch fermentation under the optimized fermentation conditions. Three repeated batch fermentation were carried out in 6 L horizontal bioreactor. The highest lipase activity of 110 U/mL and the average lipase activity of 95 U/mL were achieved. The lipase productivity increased from 2021 U/(h · L) in batch fermentation to 3476 U/(h · L) in repeated batch fermentation, which was 1. 72 times as high as that in batch fermentation. It was found that immobilized cells showed high stability for repeated use.关键词
少根根霉/脂肪酶/固定化/卧式生物反应器/重复批次发酵Key words
Rhizopus arrhizus/ lipase/ immobilization/ horizontal bioreactor/ repeated-batch fermentation引用本文复制引用
冀颐之,龚平,赵有玺,王群群,谭天伟..卧式生物反应器固定化少根根霉发酵生产脂肪酶[J].工业微生物,2012,42(6):8-13,6.基金项目
国家"863"项目(No.2002AA514030) （No.2002AA514030）
国家"十五"攻关项目(No.2001BA708B02208) （No.2001BA708B02208）
国家自然科学基金项目(No.2107600C) （No.2107600C）
中石化项目(No.202059) （No.202059）
北京市属高等学校人才强教计划资助项目-中青年骨干人才项目(PHR-201108426) （PHR-201108426）
北京市委组织部优秀人才培养资助项目(2012D005022000007). （2012D005022000007）
