工业微生物2012，Vol.42Issue(6)：42-46,5.DOI:10.3969/j.issn.1001-6678.2012.06.008
耐热马克斯克鲁维酵母发酵产乙醇研究
Ethanol fermentation by thermotolerant yeast K.marxianus
戴振鹏 1王永红 1储炬 1庄英萍 1张嗣良1
作者信息
- 1. 华东理工大学生物反应器工程国家重点实验室,上海200237
- 折叠
摘要
Abstract
Using the thermotolerant Kluyveromyces marxianus strain with semi-defined medium, the optimum ethanol fermentation temperature range was 34 ℃ ~ 40 ℃, at which the glucose conversion and ethanol productivity were over 86% and 4.4 g/(L · h) , respectively. When temperature was below 34 ℃, the fermentation period became 2-3 hours longer than that at 34 ℃ ~ 40 ℃, and the glucose conversion and ethanol productivity were 81.4% and 4.15g/(L·h), respectively. While at 45 ℃. K. Marxianus produced 30.52 g/L ethanol, 74. 8% glucose conversion and 2. 95 g/( L · h) ethanol productivity were obtained. Using steam-exploded corn stover enzymatic hydrolysate ( containing 75. 8 g/L glucose) as substrate at 30 ℃ , 37 ℃ , 45 ℃ , all the glucose conversions of 69. 1% 71. 9% , 59. 3% and the ethanol productivity of 0. 56 g/( L · h) , 0. 58 g/( L · h) , 0. 37 g/( L · h) were obviously lower than those with the semi-defined medium. When yeast extract and inorganic salts were added into the enzymatic hydrolysate, the ethanol production increased. Both at 30 ℃ and 37 ℃ glucose conversions were about 10% higher than that without adding yeast extract and inorganic salts, but the ethanol productivities did not increase at all. The results indicated that K. Marxianus had advantages over Saccharomyces cerevisiae in ethanol fermentation at above 37℃ by using steam-exploded corn stover as substrate.关键词
马克斯克鲁维酵母/耐热/乙醇/发酵/汽爆玉米秸秆Key words
Kluyveromyces marxianus/ thermotolerant/ ethanol/ fermentation/ steam-exploded corn stover引用本文复制引用
戴振鹏,王永红,储炬,庄英萍,张嗣良..耐热马克斯克鲁维酵母发酵产乙醇研究[J].工业微生物,2012,42(6):42-46,5.
