核化学与放射化学2012，Vol.34Issue(5)：316-320,5.
碘标锰卟啉及其小鼠体内分布
Radioiodine-Labeling of MnTBAP and Its Biodistribution in Mice
摘要
Abstract
To investigate the preparation of radioiodinated MnTBAP and its biodistribution in mice, MnTBAP was labeled with 131I using the iodogen method. the radiolabeled compound was characterized by polymide TLC, in which the substratum of saline was used as developing agent. Biodistribution studies were carried out in KM mice. At different times after radiopharmaceutical i. v. administration (185 kBq 131I-MnTBAP/mouse, n=6), the animals were sacrificed. Blood samples and the tissues of interested were collected, weighted and counted. The percent injected does per gram (%ID/g) was calculated for each sample. The labeling yield of 131I-MnTBAP is 96. 3% and its radiochemical purity(RCP) are 96%, 95% and 94. 5% at 2, 24 and 48 h, respectively. Biodistribution in mice demonstrates that I131I-MnTBAP is extensive, and it is metabolized mainly in liver and kidney, as indicated by their uptake of 8. 34% ID/g and 12. 23% ID/g at 5 min, 0. 34%ID/g and 0. 73%ID/g at 4 h, respectively. The results also show that 131I-IMnTBAP could not penetrate the blood-brain barrier. The activity in thyroid increases with time. In conclusion, 131I-MnTBAP is stable and it is metabolized mainly in liver and kidney, worthy of further investigation to trace the compound in vivo and in vitro.关键词
锰卟啉/放射性碘标记/体内分布Key words
MnTBAP/ radioiodinate/ biodistribution分类
医药卫生引用本文复制引用
刁尧,刘新宁,官文征,赵文瑾,刘洁,赵恂,于成国..碘标锰卟啉及其小鼠体内分布[J].核化学与放射化学,2012,34(5):316-320,5.基金项目
辽宁省自然科学基金资助项目(No 20092118) （No 20092118）
辽宁省科学计划项目(No 2009415005) （No 2009415005）
沈阳市科学技术项目(No F11-241-00) （No F11-241-00）
