核农学报2012，Vol.26Issue(9)：1271-1277,7.
赭曲霉毒素A模拟表位的筛选及免疫学鉴定
SCREENING AND IMMUNOLOGICAL CHARACTERIZATION OF THE MIMICKING EPITOPE OF OCHRATOXIN A
摘要
Abstract
To screen the mimicking epitope of Ochratoxin A from Ph. D.-7TM and establish the immunoassay for detecting OTA with it. An anti-Ochratoxin A monoclonal antibody was used as ligand, biopanning was done to screen the mimicking epitope from a Ph.D. -7TM, which was displayed as a fusion protein with the coat protein 11I of filamentous phage M13, the positive clones was identified by ELISA, and established a immunoassay for detecting OTA with the positive phages. After four rounds of panning, 22 positive clones could bind to the OTA mAb and the binding could be blocked by the free ochratoxin A. With the DNA sequencing and peptide core sequence analysis, the amino sequence of the mimicking epitope is MPLWXDL(X is any amino acid residues). A competitive ELISA immunoassay was established with the positive clones, the linear range of the inhibition curves is between 250pg/ml and 8000pg/ml. Based on the OTA mAb, the phage display technique can be successfully applied to screen the mimicking epitope of Ochratoxin A, the obtain phage can replaced Ochratoxin A to establish the immunoassay.关键词
噬菌体随机七肽库/赭曲霉毒素A/模拟表位/单克隆抗体Key words
Phage random 7 peptide library/Ochratoxin A/mimicking epitope/monoclonal antibody分类
生物科学引用本文复制引用
王耀,胡骁飞,裴亚峰,赵东,刘运超,张改平,李志西,邓瑞广..赭曲霉毒素A模拟表位的筛选及免疫学鉴定[J].核农学报,2012,26(9):1271-1277,7.基金项目
农业部公益性行业（农业）科研专项（201203040）,河南省扶持企业自主创新资金项目,河南省农业科学院科技发展及示范推广专项基金 （）
