湖南中医药大学学报2012，Vol.32Issue(11)：11-15,5.DOI:10.3969/j.issn.1674-070X.2012.11.003.011.05
心痛舒含药血清预处理对乳鼠心肌细胞缺氧/复氧损伤及TNF-α、IL-1β的影响
Effects of preconditioning with serum containing Xintongshu on the newborn rat's myocardial cells with hypoxia and reoxygenation injury and the expression of TNF-αand IL-1β
摘要
Abstract
Objective To observe the regulating effects of preconditioning with serum containing Xintongshu on the expression of nuclear factor-KBp65 (NF-KBp65), tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β), and to explore the protective mechanism of Xintongshu on the hypoxia and reoxygen injury myocardial cell model of newborn rat. Methods The myocardial cells were isolated from neonatal rats and cultured,then modelled with hypoxia and reoxygenation injury. The modeled myocardial cells were randomly divided into four groups, including normal control group, model control group, positive control group and preconditioning group treated with serum containing Xintongshu (Xintongshu group). The myocardial cell morphologic changes were observed by Rhodamine B stain. The TNF -α and IL -β proteins in culture media were detected by ELISA and the mRNA expression level of NF-KBp65 was detected by RT-PCR. Results Compared with model control group,the expression levels of TNF-α and IL-β proteins and NF-κBp65 mRNA in positive control group and Xingtongshu group were decreased. There were statistical differences (P<0.01). Compared with positive control group, the expression levels of NF-κBp65 mRNA in Xingtongshu group were statistically decreased (P<0.05). Conclusion The preconditioning with serum containing Xintongshu can exert a protective effect by inhibiting the inflammatory reaction pathway of the hypoxia and reoxygenation myocardial cells. The effect may be related to the decrease of the NF-κB p65 mRNA expression level and further inhibition of thegeneration of inflammatory cells.关键词
心痛舒/心肌细胞/炎症因子/心肌细胞核因子-κBp65/肿瘤坏死因子-α/白介素-1β/乳鼠/作用机制Key words
Xintongshu/ myocardial cell/ inflammatory cytokines/ NF-κB p65/ TNF-α/ IL-1β/ newborn rat/ mechanism of iaction分类
医药卫生引用本文复制引用
任婷,黄政德,田雪飞,陈楚淘..心痛舒含药血清预处理对乳鼠心肌细胞缺氧/复氧损伤及TNF-α、IL-1β的影响[J].湖南中医药大学学报,2012,32(11):11-15,5.基金项目
国家自然科学基金资助项目(30973750) （30973750）
湖南省教育厅重点科研项目(09A071) （09A071）
湖南省重点学科中医内科学资助项目. （）
