湖南中医药大学学报2012，Vol.32Issue(11)：19-23,5.DOI:10.3969/j.issn.1674-070X.2012.11.005.019.05
六味地黄汤对5/6肾切除大鼠肾组织中细胞外基质的影响
Effects of Liuwei Dihuang decotion on extracellular matrix of renal tissues in 5/6 nephrectomy rats
摘要
Abstract
Objective To observe the influence of Liuwei Dihuang decotion on the extracellular matrix of renal tissue in rat model with chronic renal failure and study its mechanism of preventing renal interstitial fibrosis. Methods The rat model of chronic renal failure were induced by 5/ 6 nephrectomy, rats were divided into 4 groups as follows: blank control group, sham operation group, model group and Liuwei Dihuang decotion group. At 8 weeks after the operation, index of renal function was detected, the pathological change of renal tissues was observed by HE stain, collagen deposition in renal was detected by Masson staini, the Col Ⅰ , Col Ⅲ, FN expression levels in renal tissues were examined by immunohistochemistry assay, the PDGF-A, PDGF-B protein levels in the renal tissues were examined by western blot assay. Results Compared with sham operation group, 24h urinary protein, blood urea nitrogen and serum creatinine in model group were increased significantly (P<0.01). Compared with model group, 24h urinary protein (P<0.01), blood ure-a nitrogen(P<0.01) and serum creatinine(P<0.05) in liuwei Dihuang decotion group were decreased significantly. Renal interstitial fibrosis and collagen deposition were obvious in model group, and renal interstitial fibrosis and collagen fibers deposition were markedly reduced in Liuwei Dihuang decotion group. Compared with sham operation group, the Col Ⅰ , Col Ⅲ, FN protein levels of renal tissues in model group were significantly increased (P<0.01). And these proteins expression levels in Liuwei Dihuang decotion group were significantly lower than those in model group (P<0.05). Compared with sham operation group, the PDGF-A, PDGF-B protein levels of renal tissues in model group were significantly increased(P<0.01). The expression levels of PDGF-A and PDGF-B proteins in Liuwei Dihuang decotion group were significantly lower than those in model group (P<0.01).Conclusion Liuwei Dihuang decotion may reduce the accumulation of extracellular matrix of renal tissues and prevent renal interstitial fibrosis through down-regulating the expression of PDGF-A and PDGF-B.关键词
肾间质纤维化/5/6肾切除/细胞外基质/大鼠/六味地黄汤/熟地黄/山药/山茱萸Key words
renal interstitial fibrosis/ 5/6 nephrectomy/ extracellular matrix/ rat/ Liuwei Dihuang decotion/ prepared Radix rehmanniae/ Yam/ Cornus分类
医药卫生引用本文复制引用
唐群,何泽云,徐文峰,陈丽,徐琴,曾海飞,张爽爽..六味地黄汤对5/6肾切除大鼠肾组织中细胞外基质的影响[J].湖南中医药大学学报,2012,32(11):19-23,5.基金项目
湖南省自然科学基金(重点)资助项目(09JJ3064) （重点）
湖南省高校创新平台(09K057). （09K057）
