湖南农业大学学报（自然科学版）2012，Vol.38Issue(6)：607-611,5.DOI:10.3724/SP.J.1238.2012.00607
基于SRAP标记的刺葡萄亲缘关系分析
Relationship analysis for Vitis davidii Fo(e)x with SRAP molecular markers
摘要
Abstract
Sequence-related amplified polymorphism (SRAP) molecular markers were used to detect the genetic diversity and relationship of 21 accessions of Vitis davidii Foex. From 84 pairs of primers, 7 pairs were selected out. Sixty-one clear bands were amplified using these 7 pairs of primers, of which 34 were stable morphological bands, accounting for 55.7% of total bands. Revealed by NTSYS software, the genetic similarities of 21 accessions ranged from 0.80 to 0.99. Cluster analysis showed that 21 accessions were classified into 3 cluster groups with genetic similarity of 0.92. UPGMA cluster analysis showed that there are 6 types of Vitis davidii Foex in Hunan, and we successfully deduced the suspected parents (which were lost during 3-time relocations) of Nongda No.l to No.9 which come from different regions of Vitis davidii Foex seeds by colchicine treatment.关键词
刺葡萄/亲缘关系/SRAP标记Key words
Vitis davidii Foex/ relationship/ sequence related amplified polymorphism分类
农业科技引用本文复制引用
刘昆玉,徐丰,石雪晖,钟晓红,杨国顺,倪建军..基于SRAP标记的刺葡萄亲缘关系分析[J].湖南农业大学学报（自然科学版）,2012,38(6):607-611,5.基金项目
国家农业部农业公益性行业科研项目(nyhy2x07-027) （nyhy2x07-027）
国家农业产业技术体系建设专项(nycytx-30-zt-06) （nycytx-30-zt-06）
湖南省科学技术厅重点科研项目(2009CK-2010) （2009CK-2010）
湖南省教育厅省级科技计划(成果推广计划)项目(09CY008) （成果推广计划）
