四川大学学报（医学版）2012，Vol.43Issue(6)：926-929,4.
BDNF对脑缺血大鼠肺组织MEK表达的调节
Block Effects of BDNF Antibody on MEK Expression in Lung of Rats with Brain Ischemia
摘要
Abstract
Objecitve To explore the block effect of brain-derived neurotrophic factor (BDNF) antibody on the mitogen extracellular kinase (MEK) expression in lung tissues of rats with brain ischemia. Methods Adult SD rats were divided into sham group, brain ischemia lung injury (BILI) group, and BDNF antibody treated group. Lung tissues were harvested at 3 days after operation. The distribution of MEK in the lung tissue was studied by immunbistochemistry, and the optical density values of MEK immunostaining was used to compare the difference of each group (n = 5). RT-PCR was used to determine the block effect of BDNF antibody on the level of MEK mRNA (n=8). Results Immunohistochemistry showed that MEK immunoreactive positive products were observed in endothelia cells of airway and blood vessels. The optical density value in rats with brain ischemia significantly increased, compared with sham one (P<0. 05). Moreover, BDNF antibody block could reverse this increase of BDNF immunostaining intensity (P<0. 05). RT-PCR showed that BDNF antibody block significantly decreased the expressional level of MEK mRNA in the lung tissues with brain ischemia (P<0. 05). Conclusion BDNF antibody block leads to a significant reduction of MEK expression in endothelia cells of lung tissue in the rats with brain ischemia.关键词
脑缺血肺损伤/大鼠/BDNF抗体/丝裂原细胞外激酶/肺组织Key words
Brain ischemia lung injury/Rat/BDNF antibody block/MEK/Lung tissue引用本文复制引用
余琳,但齐琴,李劲涛,张云辉..BDNF对脑缺血大鼠肺组织MEK表达的调节[J].四川大学学报（医学版）,2012,43(6):926-929,4.基金项目
云南省自然科学基金(No.2009CD199)资助 （No.2009CD199）
