华中师范大学学报（自然科学版）2012，Vol.46Issue(6)：744-750,7.
偏肿革裥菌漆酶基因克隆及不同染料脱色研究
Cloning and different dye decolorization of a laccase gene from white rot fungus Lenzites gibbosa
摘要
Abstract
Dyes are usually difficult to be decolorized due to their complex chemical structures. Chemical different dyes were used in the dye decolorization test. Laccase crude enzyme solution to a final concentration of 50 mg / L of different chemical structure of the dye decolorization, And fast grace quinones Alizarin Red decolorization of up to 100% ; Heterocyclic neutral red, Azo kind of Congo red and Triphenylmethane crystal violet Decolorizing effect below alizarin red. The result demonstrated the potential application prospects of the laccase from Lenzites gibbosa dye-containing wastewater treatment. Using PCR and RACE technique, We obtain the cDNA and genomic DNA sequence of the laccase gene from Lenzites gibbosa, Genome size for 2 106 bp. By comparing the laccase cDNA gene and genome DNA sequence length, Found that the gene contains 11 exon and 10 introns. cDNA sequence of full length for 1 982 bp, contains a complete ORF, length for 1 563 bp, code 520 amino acids.关键词
偏肿革裥菌/漆酶基因/染料脱色/结构域Key words
Lenzites gibbosa / laccase gene/ dye decolorization/ function domain分类
生物科学引用本文复制引用
郑苗苗,池玉杰..偏肿革裥菌漆酶基因克隆及不同染料脱色研究[J].华中师范大学学报（自然科学版）,2012,46(6):744-750,7.基金项目
国家自然科学基金项目(30671700) （30671700）
中国博士后科学基金项目(20090460866). （20090460866）
