基础医学与临床Issue(9)：1059-1063,5.
筋脉通含药血清降低高糖培养大鼠雪旺细胞活性氧水平及PARP-1蛋白表达
Effects of Chinese herbal medicine Jinmaitong-containing serum on the ROS level and expression of PARP-1 of rat Schwann cells cultured in high-glucose medium
摘要
Abstract
Objective To investigate the effects of medicated serum prepared by administration of Jinmaitong (JMT) , a compound Chinese herbal medicine, on oxidative damage and poly ADP-ribose polymerase-1 (PARP-1) of Schwann cells cultured in high-glucose medium. Methods SD rats were divided into normal control group ( distilled water) ,JMT group and vitamin C group to prepare medicated serum. Bilateral sciatic nerves of new born SD rats were used to separate Schwann cells. Schwann cells cultured in high-glucose medium were divided into high glucose group (50 mmol/L glucose medium,JMT group( JMT-medica-ted serum) and vitamin C( VC) group (VC-medicated serum). Schwann cells cultured in DMEM were used as the normal control. After48 h culturing, the level of ROS was measured by confocal laser scanning microscope with 2',7'-dichlorofluorescein( DCF) as a molecular probe and the expression of PARP-1 protein was detected by Western blot. Results 1) Compared with high glucose group, the fluorescence intensities of ROS-DEC in Schwann cells cultured in JMT and VC groups were weaker significantly (P <0. 01). There were no significant differences between these two treated groups. 2) Compared with high glucose group,the expression of PARP-1 (89 ku) in Schwann cells cultrued in JMT group decreased significantly (P <0. 01). The expression of JMT group was also much lower than that of VC group (P <0. 01). Conclusions The medicated serum of JMT down-regulates the expression of ROS and PARP-1 of Schwann cells cultured in high glucose medium and reduces the oxidative DNA damage.关键词
雪旺细胞/高糖/氧化应激损伤/PARP-1/筋脉通Key words
Schwann cell/ high glucose/ oxidation damage/ PARP-1 / Jinmaitong capsule分类
医药卫生引用本文复制引用
石玥,梁晓春,张宏,王普艳,赵丽..筋脉通含药血清降低高糖培养大鼠雪旺细胞活性氧水平及PARP-1蛋白表达[J].基础医学与临床,2012,(9):1059-1063,5.基金项目
北京市自然科学基金(7082077) （7082077）
