基础医学与临床2012，Vol.32Issue(11)：1293-1297,5.
ATF4基因在小鼠胚胎围着床期子宫内膜的表达
Expression of ATF4 gene in mouse endometrium during peri-implantation process of embryos
摘要
Abstract
Objective To investigate the expression of activating transcription factor4 (ATF4) gene in mouse endometrium during peri-implantation process of embryos, and the role in the embryonic implantation. Methods Endometrium samples were collected from Day 0 to Day 7 in early pregnant mice, and the express level of ATF4 mRNA on it was determined by RT-PCR and the expression level of ATF4 protein as determined by immunohistochemistry and Western blot. Polyclonal antibody of ATF4 was injected into the uterus of the early pregnant mice to observe the changes of the embryos. Results 1The expression of ATF4 on the endometrium of pregnant mice was stronger than that of nonpregnant mice(P <0. 05) , and the tendency was gradually augmented as days went. It reached the highest level at pregnant d 4 and declined gradually thereafter. 2 ) The clearest expression of ATF4 protein was primarily limited to the subluminal stromal cytoplasm in the endometrium of mouse. Expressional mode of ATF4 protein was similar with that of the mRNA. 3)ATF4 antibody resulted in a significant decrease of embryo implantation as compared with the control group. Conclusions ATF4 gene may be involved in the decidualization, and promote the embryo implantation.关键词
ATF4/着床/子宫内膜/蜕膜化Key words
ATF4/implantation/ endometrium/decidualization分类
医药卫生引用本文复制引用
柴路维,姜蓉,何小茜,黄燕,杨戎..ATF4基因在小鼠胚胎围着床期子宫内膜的表达[J].基础医学与临床,2012,32(11):1293-1297,5.基金项目
重庆市教委课题(KJ110307) （KJ110307）
