基础医学与临床2012，Vol.32Issue(11)：1332-1336,5.
超声辐照微泡对肝细胞生长因子转染大鼠损伤面神经的促进作用
Hepatocyte growth factor loaded ultrasound microbubble contrast facilitates the restoration of injuried facial nerve of rats
郝亚宁 1骆文龙1
作者信息
- 1. 重庆医科大学附属第二医院耳鼻咽喉头颈外科,重庆400010
- 折叠
摘要
Abstract
Objective To explore the feasibility and efficacy of therapeutic role in injuried facial nerve induced by Hepatocyte growth factor ( HGF) mediated by ultrasound-targeted microbubble destruction. Methods Forty SD rats were divided into 4 groups after the models of facial nerve injury were established: A, PBS group, B, HGF and mi-crobuble (HGF + MB) group, C, HGF and ultrasound (HGF + US) group, D, HGF + ultrasound + microbubble (HGF + MB/US)group. The nerve conduction velocity(NCV) and potential latency and amplitude of facial nerve were measured on 28 d in all rats after transfection. HGF expression was detected by Western blot. HGF mRNA expression was detected by real-time RT-PCR. Results The behavioral analyses results showed that the nose tip position and vibrissae movement in D group were better than those of other groups. The NCV and potential amplitude of the facial nerve in D group were increased, but the potential latency was decreased. HGF expressions was higher than those of other groups in D gouop. The expression of HGF mRNA in D group was significantly higher than that of the other groups (P <0. 05). Conclusions Ultrasound-mediated mircobuble destruction may improve the transfection of HGF in injury facial nerve safely and facilitates its restoration.关键词
超生微泡/肝细胞生长因子/面神经损伤Key words
ultrasound mircobubble/ hepatocyte growth factor/ facial nerve injury分类
医药卫生引用本文复制引用
郝亚宁,骆文龙..超声辐照微泡对肝细胞生长因子转染大鼠损伤面神经的促进作用[J].基础医学与临床,2012,32(11):1332-1336,5.
