基础医学与临床2012，Vol.32Issue(11)：1337-1341,5.
登革病毒4型E蛋白的表达与多克隆抗体的制备
Expression of the envelope protein of Dengue virus type 4 and preparation of the polyclonal antibody
李竹石 1俞永新 2李玉华 2林华 1杨健 1杨会强 1曾献武 1赵宇 1刘俐 1刘莉娜 1康庄1
作者信息
- 1. 中国生物技术集团公司成都生物制品研究所有限责任公司,四川成都610023
- 2. 中国食品药品检定研究院,北京100050
- 折叠
摘要
Abstract
Objective To express full length (DENV4E) and domain III (DENV4EIII) of the envelope protein of Dengue virus type 4 in procaryotic cells respectively, and to prepare the polyclonal antibody against dengue virus by immunizing rabbits with the expressed proteins. Methods The sequences encoding DENV4E and DENV4EIII proteins were cloned into pET-32a ( + ) plasmids respectively to construct expression vectors. Then DENV4E or DENV4EIII proteins were expressed in Rosetta cells by IPTG induction and detected by SDS-PAGE. Polyclonal antibody against DENV4E or DENV4EIII proteins was obtained by immunizing rabbits with the expressed proteins and was identified by Western blot. Results DENV4E and DENV4EIII proteins were both successfully expressed in Rosetta cells mainly in the form of inclusion body. The prepared polyclonal antibody formed bands as expected when reacting with the dengue virus. Conclusions The polyclonal antibody is prepared and may be used in the detection of dengue virus.关键词
登革病毒4型/E蛋白/多克隆抗体Key words
dengue virus type 4/ envelope protein/ polyclonal antibody分类
生物科学引用本文复制引用
李竹石,俞永新,李玉华,林华,杨健,杨会强,曾献武,赵宇,刘俐,刘莉娜,康庄..登革病毒4型E蛋白的表达与多克隆抗体的制备[J].基础医学与临床,2012,32(11):1337-1341,5.
