基础医学与临床2012，Vol.32Issue(12)：1378-1383,6.
不同临床类型原发鼻咽癌组织差异表达蛋白
Differentially expressed proteins in the primary focus of nasopharyngeal carcinoma in different clinical types
摘要
Abstract
Objective To explore the differentially expressed protiens of the tumor tissues of nasopharyngeal carcinoma ( NPC ) primary focus in different clinical types including upward progressing type, downward progressing type and mixed progressing type. Methods The total proteins of tissues from normal nasopharyngeal mucosa and NPC in different clinical types were separated by two-dimensional electrophoresis (2-DE) , then proteins spots were analyzed and the differentially expressed proteins were identified by matrix assisted laser desorption/ionization time-offlight mass spectrometry ( MALDI-TOF-MS) combined with bioinformatics analysis. Results Thirty-one significant differentially expressed proteins between mixed progressing type of NPC and normal tissues were found. Eighteen proteins were up-regulated and 13 proteins were down-regulated in mixed type of NPC. Five proteins of ten differential expressed proteins selected from mixed progressing type of NPC were identified by MALDI-TOF-MS. Heat shock cognate 71 kDa protein,alpha-1-antitrypsin and macrophage-capping protein were up-regulated and Protein S100A9 and Protein 4. 1 were down-regulated in mixed progressing type of NPC. Thirty-one significant differentially expressed proteins between upward and downward progressing types of NPC were also found. And among of them, 15 proteins were up-regulated and 16 proteins were down-regulated in upward progressing type of NPC as compared with those in downward progressing type of NPC. Six proteins of ten differential expressed proteins selected from upward progressing types of NPC were identified by MALDI-TOF-MS. Mitochondrial aconitate hydratase, alcohol de-hydrogenase [ NADP + ] and Rab GDP dissociation inhibitor beta were up-regulated and NM23-H1, nuclear chloride channel-27 and aldehyde dehydrogenase X were down-regulated in upward progressing type of NPC as compared with those in downward progressing type of NPC. Conclusions There were significant differences at proteins related to tumor metastasis, energy metabolism,retoxity and anti-metastasis between different clinical type of NPC. These proteins may function as markers of clinical types of NPC.关键词
鼻咽癌/临床类型/蛋白质组学/质谱分析/差异Key words
nasopharyngeal carcinoma/ clinical types/ proteomics/ mass spectrometry/ difference分类
医药卫生引用本文复制引用
何迎春,陈艳,廖端芳,何兰,林丽美,范婧莹,田道法..不同临床类型原发鼻咽癌组织差异表达蛋白[J].基础医学与临床,2012,32(12):1378-1383,6.基金项目
国家自然科学基金(30973856,81072904,81273988) （30973856,81072904,81273988）
中国博士后科学基金(2011M501277) （2011M501277）
湖南省科技计划项目(2010SK3021) （2010SK3021）
湖南省2011年研究生创新基金研究项目(CX2011B335) （CX2011B335）
湖南省中医五官科学重点学科 （）
