基础医学与临床2012，Vol.32Issue(12)：1450-1455,6.
靶向SARS-CoV spike中和抗体的检测
Targeting SARS-CoV spike neutralizing antibody test
马萍 1杨宁 1郎建设 1蒋澄宇1
作者信息
- 1. 中国医学科学院基础医学研究所,医学分子生物学国家重点实验室,北京10005
- 折叠
摘要
Abstract
Objective To establish a targeting SARS-CoV spike neutralizing antibody test model. Methods Construction of optimal whole SARS-CoV spike gene plasmid to induce mouse neutralizing antibody. Pretreatment with S318-510 protein targeting ACE2 to observe the inhibition change of SARS-CoV S pseudoviruses infection process via fluorescence microscope. Results _ SARS-CoV SI 190 Abs prevented SARS-CoV pseudovirus entry into HEK293E/ACE2-Myc cells and pretreatment with S318-510 inhibited neutralization of SARS-S entry antiserum. Conclusions This test model confirmation of SARS-CoV RBD targeting SARS-CoV spike neutralizing antibody may function as a test model of lethal viruses in laboratory not equiped as biosafety level 3.关键词
SARS-CoV/假病毒/中和抗体Key words
SARS-CoV/ pseudo-virus/ neutralizing antibody分类
医药卫生引用本文复制引用
马萍,杨宁,郎建设,蒋澄宇..靶向SARS-CoV spike中和抗体的检测[J].基础医学与临床,2012,32(12):1450-1455,6.
