解放军医学杂志2012，Vol.37Issue(11)：1054-1058,5.
乳腺癌MDA-MB-231细胞源Exosomes对内皮细胞增殖的影响及MAPK/ERK和PI3K/Akt信号通路的作用
Effects of exosomes derived from MDA-MB-231 on proliferation of endothelial cells and the role of MAPK/ERK and PI3K/Akt pathways
隆霜 1沈宜 1谢莹珊 1范维珂 1姜蓉 1陈黎1
作者信息
- 1. 400016 重庆医科大学基础医学院病理生理教研室,干细胞与组织工程研究室
- 折叠
摘要
Abstract
Objective To investigate the effects of exosomes derived from breast cancer cell line MDA-MB-231 on proliferation of human umbilical cord vein endothelial cells (HUVECs), and evaluate the role of MAPK/ERK and PI3K/Akt signal transduction pathway during the process. Methods Exosomes were derived and purified from MDA-MB-231 by cryogenic ultracentrifugation and density gradient centrifugation. MTT assay was carried out for measurement of cell proliferation in HUVECs with exosome of 50, 100, 200 and 400μg/ml. The states of cell cycle of HUVECs co-cultured with 200μg/ml exosomes were detected by flow cytometry. The effects of 200μg/ml exosomes on the expression of ERK, Akt and phosphorylated ERK,Akt in HUVECs were detected with Western blotting. Results Exosomes derived from MDA-MB-231 significantly promoted HUVECs proliferation in a classical time- and dose-dependent manner. Flow cytometry revealed that, co-cultured with 200μg/ml exosomes for 24h, S-phase cells in HUVECs increased, while G1/S phase cells in HUVECs decreased. Western blotting showed that, co-cultured with 200μg/ml exosomes for 24h, 48h and 72h, the expressions of phosphorylated ERK and Akt were up-regulated in a time-dependent manner. Conclusion Exosomes derived from breast cancer cell line MDA-MB-231 may promote HUVECs proliferation, the changes in cell cycle and the continuous activation of the MAPK/ERK and PI3K/Akt signal transduction pathways may be the underlying mechanism.关键词
Exosomes/胞外信号调节激酶/蛋白激酶B/增殖/乳腺癌Key words
Exosomes/ extracellular signal regulatory kinase/ Akt/ proliferation/ breast cancer
分类
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隆霜,沈宜,谢莹珊,范维珂,姜蓉,陈黎..乳腺癌MDA-MB-231细胞源Exosomes对内皮细胞增殖的影响及MAPK/ERK和PI3K/Akt信号通路的作用[J].解放军医学杂志,2012,37(11):1054-1058,5.
