实用临床医药杂志2012，Vol.16Issue(23)：28-31,4.
肿瘤坏死因子-α与骨桥蛋白在类风湿关节炎滑膜成纤维细胞中表达的相互影响及其信号转导途径
Interaction and signal transduction pathways of tum or necrosis factor-α and osteopontin in synovial fibroblasts of rheumatoid arthritis
摘要
Abstract
Objective To analyze the interaction and possible signal transduction pathways of tumor necrosis factor-α (TNF-α) and osteopontin ( OPN) in expression of synovial fibroblasts of rheumatoid arthritis. Methods Collagen-induced arthritis (CIA) model was induced by bovine II collagen and incomplete Freund's adjuvant. The synovial membrane of the normal control group and model group were taken to culture synovial fibroblasts cells (SFs) by collagenase digestion. The SFs were divided into control group, model group, model group + lipopolysaccharide ( LPS) , model group + LPS + p38 channel inhibitor (SB203580). The levels of OPN mRNA were evaluated, and the interaction and possible signal transduction pathways between TNF-a and OPN were analyzed. Results LPS significantly increased the expression of OPN mRNA in model group cells (P <0. 01 ) , the addition of SB203580 significantly reduced the expression of OPN mRNA. There were significant differences among the 4 groups (P <0.01). Conclusion TNF-a can induce the expression of OPN, and this process are able to realize through p38 mitogen activated protein kinase (p38MAPK) signal transduction pathway.关键词
胶原诱导性关节炎/滑膜成纤维细胞/肿瘤坏死因子-α/骨桥蛋白/p38丝裂原活化蛋白激酶Key words
collagen-induced arthritis/synovial fibroblasts cells/tumor necrosis factor-α/osteopontin/p38 mitogen activated protein kinase分类
医药卫生引用本文复制引用
黄文辉,吕志芬,陶怡,黄成辉,陈瑞林,张北平..肿瘤坏死因子-α与骨桥蛋白在类风湿关节炎滑膜成纤维细胞中表达的相互影响及其信号转导途径[J].实用临床医药杂志,2012,16(23):28-31,4.基金项目
广东省医学科研基金资助项目(A2008282) （A2008282）
中国高校医学期刊床专项资金(11220243) （11220243）
