江西农业大学学报2012，Vol.34Issue(5)：1039-1042,1048,5.
药用真菌桑黄的液体发酵培养基的配方优化筛选研究
Optimal Screening of Medium Formula for Liquid Fermentation Culture of Medicinal Fungus Phellinus igniarius
摘要
Abstract
The current study aims to explore a favorable formula for the shaking flask fermentation culture of the valuable medicinal fungus Phellinus igniarius. Based on the single factor test results of different concentrations and different carbon sources, nitrogen sources, inorganic salts using corn flour, soybean flour and KC1 the L9 (34) orthogonal shaking - flask culture of three factors and three levels was carried out at the speed of 135 r/min, with a period of 9 days at the temperature of 28℃. The fermentation medium optimization was based on the mycelium dry weight for the detection formula. A favorable formula for the shaking flask fermentation culture was obtained preliminarily, which contained corn powder 30 g/L, soybean powderlO g/L, KCl 2 g/L, KH2PO4 4 g/L, MgSO4 2 g/L, VB1 0. 1 g/L, and Phellinus igniarius mycelium dry weight was up to 2.23 g/100mL in the shaking flask fermentation culture condition. A favorable formula for the shaking flask fermentation culture of Phellinus igniarius was obtained preliminarily, which laid a foundation for further research of Phellinus igniarius in shaking flask fermentation condition.关键词
桑黄/发酵培养基/优化筛选Key words
Phellinus igniarius/ fermentation medium/ optimal screening分类
生物科学引用本文复制引用
傅海庆,周阳,傅华英..药用真菌桑黄的液体发酵培养基的配方优化筛选研究[J].江西农业大学学报,2012,34(5):1039-1042,1048,5.基金项目
福建省教育厅A类项目(JA12378)和福建农林大学青年基金资助项目(2009057) （JA12378）
