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大豆富硒灵芝菌固体发酵产氨基酸工艺的优化研究

杜亚楠 涂晓嵘 胡志斌 李庆蒙 魏赛金

江西农业大学学报2012,Vol.34Issue(5):1043-1048,6.
江西农业大学学报2012,Vol.34Issue(5):1043-1048,6.

大豆富硒灵芝菌固体发酵产氨基酸工艺的优化研究

Optimization of the Solid Fermentation Soybean Enrich Selenium Ganoderma Producing Amino Acid

杜亚楠 1涂晓嵘 1胡志斌 1李庆蒙 1魏赛金1

作者信息

  • 1. 江西农业大学生物科学与工程学院/南昌市发酵应用重点实验室,江西南昌330045
  • 折叠

摘要

Abstract

To explore the conditions for the solid fermentation to produce free amino acids by inoculating Se-enriched Ganoderma liquid seed in soybean solid medium. On the basis of exploring better inoculation a-mount, incubation temperature and fermentation period, the response surface method of central composite design test was used. Ideal combination conditions for Se-enriched Ganoderma On soybean solid fermentation culture; inoculating Se-enriched Ganoderma liquid seed in soybean solid medium, inoculation amount 10.53% , fermentation temperature 29. 34 ℃, fermentation period 6. 68 d, free amino acids in fermented soybean up to 280.56 mg/100g, improved by 64.64% compared with those before optimization, organic selenium content up to 0.036% ,1.1 times more than before optimization. Process conditions for producing free amino acid from Ganoderma in soybean solid fermentation has initially explored, that provides new ideas of developing soybean enrich free amino acid and trace organic selenium.

关键词

富硒灵芝/氨基酸/响应面法

Key words

Ganoderma lucidum enrich selenium/ dissociative amino acid/ response surface method

分类

农业科技

引用本文复制引用

杜亚楠,涂晓嵘,胡志斌,李庆蒙,魏赛金..大豆富硒灵芝菌固体发酵产氨基酸工艺的优化研究[J].江西农业大学学报,2012,34(5):1043-1048,6.

基金项目

江西省教育厅项目(GJJ08193) (GJJ08193)

江西农业大学学报

OA北大核心CSCDCSTPCD

1000-2286

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