昆虫学报2012，Vol.55Issue(9)：1082-1092,11.
DNA 条形码技术在北京百花山地区夜蛾科物种鉴定中的应用
Species identification of Noctuidae moths ( Insecta: Lepidoptera ) from Baihuashan, Beijing, China with DNA barcoding
摘要
Abstract
To explore the feasibility of DNA barcoding in the identification of Noctuidae moths, the COI genes of 75 samples belonging to 43 species from Noctuidae in Baihuashan, Beijing, were amplified using universal barcoding primers. The intraspecies and interspecies genetic distances were calculated using the Kimura-2-parameter model, the phylogenetic trees were reconstructed by neighbor-joining ( NJ ) and maximum parsimony ( MP) method, and the identification of MOTU was performed using sequence divergence threshold technique. The results showed that all of Noctuidae species were successfully distinguished by the phylogenetic tree. The average intraspecific genetic distance (0. 02% ) was much less than the average interspecific genetic distance (11. 28%). Seventy-five Noctuidae samples were divided into 42 MOTUs based on 1% sequence divergence threshold and the accuracy rate was 95%. Most of MOTUs (41/42) were classified into their corresponding morphospecies with only one exception MOTU04 The study indicates that the species from Noctuidae familv can be well distinguished with the commonly used COI barcodes, which are potentially well utilized in moth species identification.关键词
鳞翅目/夜蛾/分子分类/DNA条形码/系统发育树/遗传距离/分子可操作分类单元Key words
Lepidoptera/ noctuid/ molecular taxonomy/ DNA barcoding/ phylogenetic tree/ genetic distance/ molecular defined operational taxonomic unit ( MOTU )分类
生物科学引用本文复制引用
杨聪慧,韩辉林,迟美妍,金倩,武春生,朱朝东,张爱兵..DNA 条形码技术在北京百花山地区夜蛾科物种鉴定中的应用[J].昆虫学报,2012,55(9):1082-1092,11.基金项目
北京市自然科学基金重点项目(KZ201010028028) （KZ201010028028）
北京市人才强教项目(PHR201107120) （PHR201107120）
国家自然科学基金项目(31071963,31272340, 31272355) （31071963,31272340, 31272355）
