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两广地区家蚕白僵菌的SSRs遗传多样性

吕思行 刘吉平 汤历 李香霖 米红霞 杨吉龙 晏育伟 徐树兰

昆虫学报2012,Vol.55Issue(10):1142-1148,7.
昆虫学报2012,Vol.55Issue(10):1142-1148,7.

两广地区家蚕白僵菌的SSRs遗传多样性

Genetic diversity of Beauveria bassiana in different silkworm populations from Guangdong and Guangxi,southern China,based on SSRs

吕思行 1刘吉平 1汤历 2李香霖 1米红霞 1杨吉龙 1晏育伟 1徐树兰2

作者信息

  • 1. 华南农业大学动物科学学院,广东省农业动物基因组学与分子育种重点实验室,广州 510642
  • 2. 广州市生物防治站,广州 510460
  • 折叠

摘要

Abstract

The genetic diversity of 13 Beauveria bassiana strains in different silkworm (Bombyx mori) populations from Guangdong and Guangxi, southern China, was analyzed based on simple sequence repents (SSRs) A total of 137 fragme were obtained with 9 SSR primers, on acerage 15 fragments (Gst) was 0.0590. the polymorphic rate(PPL) was 97.73% the Nei's genetic diversity index ( H )was 0. 1896, and the Shannon' s information diversity index (1) was 0. 3165. The level of genetic variation of Guangdong populations was higher (PPL =68. 18% , H = 0. 1910, I=0. 3044) , while that of the Guangxi populations (PPL=65.91% , H = 0. 1713. 7=0. 1791) is smaller. The result showed that the genetic diversity of B. bassiana Guangdong strains was higher than that of B. bassiana Guangxi strains. The dendrogram of B. bassiana Guangdong and Guangxi strains was constructed by UPGMA cluster analysis based on the Nei' s genetic distance, and it is found that different strains from different collecting localities are divided into two clades. The results indicate that the genetic structure of B. bassiana from Guangdong and Guangxi is very complicated, and there is certain degree of similarity between the strains of Beauveria from the partial silkworm region, suggesting that it is feasible to trace the origin of B. bassiana with SSRs

关键词

家蚕/白僵病/白僵菌/微卫星标记/遗传多态性

Key words

Bombyx mori/ white muscardine/ Beauveria bassiana/ simple sequnce repeats ( SSRs) / genetic diversity

分类

生物科学

引用本文复制引用

吕思行,刘吉平,汤历,李香霖,米红霞,杨吉龙,晏育伟,徐树兰..两广地区家蚕白僵菌的SSRs遗传多样性[J].昆虫学报,2012,55(10):1142-1148,7.

基金项目

现代农业产业技术体系建设专项(蚕桑)(nycytx-27-gw202) (蚕桑)

广东省科技计划项目(2008A024100002) (2008A024100002)

广东省科技基础条件建设项目(2011B060300008) (2011B060300008)

昆虫学报

OA北大核心CSCDCSTPCD

0454-6296

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