临床神经病学杂志2012，Vol.25Issue(5)：350-353,4.
脑出血大鼠血肿周围脑组织含水量与基质金属蛋白酶-9、组织基质金属蛋白酶抑制剂-1及谷氨酸表达水平的关系
Relationship between brain water content and the expression levels of matrix metalloproteinase-9, tissue inhibitor of metalloproteinase-1 and glutamate around the hematoma in rats with intracerebral hemorrhage
摘要
Abstract
Objective To investigate the relationship between brain water content and the expression levels of matrix metalloproteinase-9 (MMP-9) , tissue inhibitor of metalloproteinase-1 ( TIMP-1) and glutamate (Glu) around the hematoma in rats with intracerebral hemorrhage (ICH). Methods Forty-eight male Wistar rats were divided into normal control group (n = 8) , sham operation control group ( n = 8 ) and ICH group ( n = 32) randomly. The ICH groups were divided into ICH 12 h,24 h,72 h and 7 d 4 subgroups, and every subgroup has 8 rats. The ICH rat models were mad with injected the autologous blood to caudate nucleus. The brain water content was measured used the wet and dry weight method,and the expressions of MMP-9, TIMP-1, Glu of brain tissue around the hematoma was examined by immunohistochemical method. Results The water content and expressions of MMP-9,TIMP-1 and Glu of all the ICH subgroups were obviously increased than those in the normal control group and sham operation control group( all P < 0.01). Among all the ICH subgroups, the MMP-9, TIMP-1 expressions got the peak at the 72 h( P < 0. 01) , while the Glu level was highest at the 24 h(P<0.01). There were positive correlations between moisture content of brain tissues (Y) and the expressions of MMP-9 (X1) and Glu (X3) , and the multiple regression equation was Y = 68. 894 + 0.281X1-0.052X3. Conclusions The water content and the expressions of MMP-9, TIMP-1 and Glu were significantly increased at the brain tissue around the hematoma after ICH. The MMP-9 and Glu played important roles in the occurrence and development of hydrops brain tissue around the hematoma after ICH.关键词
脑出血/脑组织含水量/基质金属蛋白酶-9/组织基质金属蛋白酶抑制剂-1/谷氨酸Key words
intracerebral hemorrhage/ brain water content/ matrix metalloproteinase-9/ tissue inhibitor of metalloproteinase-1/ glutamate分类
医药卫生引用本文复制引用
贺燕,戴宏伟,姬林,蔡敏,王兴臣..脑出血大鼠血肿周围脑组织含水量与基质金属蛋白酶-9、组织基质金属蛋白酶抑制剂-1及谷氨酸表达水平的关系[J].临床神经病学杂志,2012,25(5):350-353,4.基金项目
山东省2007-2008年度中医药科技发展计划(2007-100) （2007-100）
