临床神经病学杂志2012，Vol.25Issue(5)：354-357,4.
大鼠脂肪干细胞来源的施万细胞样细胞髓鞘形成能力的研究
Investigation of myelin formation ability in Schwann cell-like cells from rats adipose-derived stem cells
摘要
Abstract
Objective To investigate the myelin formation ability of Schwann cell (SC)-like cells from rats adipose-derived stem cells (ADSC). Methods Rats ADSC were harvested, cultured and identified from rats fat tissue. Heregulin, basic fibroblast growth factor, platelet-derived growth factor and forskolin were used to induce rats ADSC into SC-like cells, which were then co-cultured with PC12 cells. The morphology of SC-like cells was observed under phase contrast (×100); immunocytochemistry was used to detect the surface markers of SC-like cells; transmission electron microscope was used to identify myelin formation ability of SC-like cells. Results Rats ADSC expressed mesodermal marker fibronectin, only few cells expressed neural stem cell marker nestin. SC-like cells from rats ADSC were bi- or tri- polar in shape, positively immunostained for SC markers glial fabrilary acidic protein and S100. Electron microscope results showed that SC-like cells could form myelin structures with PC12 cells in vitro. Conclusion SC-like cells from rats ADSC could form myelin in vitro.关键词
脂肪干细胞/施万细胞/髓鞘Key words
adipose-derived stem cells/ Schwann cells/ myelin分类
医药卫生引用本文复制引用
许勇峰,刘岚..大鼠脂肪干细胞来源的施万细胞样细胞髓鞘形成能力的研究[J].临床神经病学杂志,2012,25(5):354-357,4.基金项目
国家自然科学基金(30900463) （30900463）
浙江省医药卫生优秀青年科技人才专项基金(2009QN013) （2009QN013）
