林业科学2012，Vol.48Issue(11)：130-133,4.
利用生物反应器接触培养法增殖笃斯越橘丛生苗
Proliferation of Adventitious Shoots of Vaccinium uliginosum Using Raft Bioreactors
摘要
Abstract
In order to screen culture conditions of a bioreactor for multiplying blueberry ( Vaccinium uliginosum ) , the raft culture method was used in an air-lift bioreactor to study effects of inoculation density, air volume, and light intensity on proliferation and growth of shoots. The results indicated that a mass of shoots were able to be produced by using raft culture in a bioreactor. Inoculation density affected multiplication of blueberry, 60 inoculates in a 5 L bioreactor were the most suitable inoculation density, which led 38. 6 adventitious shoots per explant, and total 2 316 vigorous shoots per bioreactor were obtained. Air volume rate of 100 mL · min had the best effect on proliferation, and it produced significantly higher growth of shoots, more shoot numbers, greater fresh and dry mass than did the air volume groups of 50 and 150 mL·min-1. Multiplication of shoots was also affected by light intensity, and 45 μmol· m -2s-1 was better than either 30 or 60 μmol · m-2 s-1 for producing dark green and vigorous shoots in the bioreactor.关键词
笃斯越橘/组织培养/生物反应器/接种量/通气量/光强Key words
blueberry ( Vaccinium uliginosum ) / tissue culture/ bioreactor/ inoculation density/ air volume/light intensity分类
农业科技引用本文复制引用
李铁军,朴炫春,廉家盛,廉美兰..利用生物反应器接触培养法增殖笃斯越橘丛生苗[J].林业科学,2012,48(11):130-133,4.基金项目
国家自然科学基金项目"生物反应器内铁皮石斛圆球茎生长动力学及有效物质积累规律的研究"(31160079). （31160079）
