南京林业大学学报（自然科学版）2012，Vol.36Issue(6)：13-16,4.
杂种鹅掌楸体胚苗根尖染色体核型分析
Karyotype analysis of the Liriodendron chinense × L.tulipifera root tip chromosome
摘要
Abstract
Ice-water mixture,0. 7 mmol/L cycloheximide and 0. 2 % colchicine were used as pretreated reagent for Liriodendron hybrids root tips of test-tube plantlet by different time grads. Then the root tips were squashed with Carbol fuch-sin and observed with microscope. It had been proved that all these three pretreatments of ice-water mixture for 6 h, 0. 7 mmol/L cycloheximide for 2 h, and 0. 2 % colchicine for 4 h could obtain metaphase chromosome specimen, but by comparison, root tips pretreated with cycloheximide and colchicine could give much better chromosome morphology. Then Karyotype of the Liriodendron chinense x L. Tulipifera was studied with chromosome samples prepared from root tip by 0. 7 mmol/L cycloheximide for 2 h. The result showed that there were 38 chromosome in Liriodendron chinense x L. Tulipifera and its karyotype formula was 2n = 38 = 26m + 8sm + 2st + 2t and the karyotypes of Liriodendron chinense x L. Tulipifera belong to category 2B of Stebbins.关键词
杂种鹅掌楸/染色体/核型分析Key words
Liriodendron chinense x L. Tulipifera/ chromosome/ karyotype analysis分类
农业科技引用本文复制引用
刘光欣,陈金慧,孙杰,愈天驰,施季森..杂种鹅掌楸体胚苗根尖染色体核型分析[J].南京林业大学学报（自然科学版）,2012,36(6):13-16,4.基金项目
国家林业局"948"引进项目(2009-4-24) （2009-4-24）
江苏省自然科学基金项目攀登计划(BK2008051) （BK2008051）
国家自然科学基金项目(30901156) （30901156）
江苏省高校自然科学基金项目(09KJA220001) （09KJA220001）
