南京林业大学学报（自然科学版）2012，Vol.36Issue(6)：90-94,5.
耐热α-葡萄糖苷酶的诱导条件及重组酶的固定化
Inducement condition and immobilization of thermostable α-glucosidase produced by Escherichia coli
摘要
Abstract
Several factors influencing a-glucosidase production with the recombinant Escherichia coli haroring the JM109/pBV-220-GLZ were investigated. The results showed that the best inducement conditions were as follows: induction temperature 40 X., induction time 14 h, and at OD600 of 2. 0. To determine the optimum conditions of immobilization, the concentration of sodium alginate, CaCl2, and glutaraldehyde, and solidifying time were studied. The results showed that the optimum operations were; 20 g/L sodium alginate, 20 g/L CaCl2,1.0% glutaraldehyde, solidifying time 2 h. Under the optimum condition, the recovery rate reached 81 %. After 15 times repeated operations, the enzyme activity remained 75 % , showing good operational stability. Thus, the superior stability of the recombinant enzyme laid the base for large-scale application.关键词
耐热α-葡萄糖苷酶/诱导条件/酶的固定化Key words
thermostable a-glucosidase/ inducement condition/ immobilization of enzyme分类
化学化工引用本文复制引用
裴建军,吕香香,解静聪,赵林果..耐热α-葡萄糖苷酶的诱导条件及重组酶的固定化[J].南京林业大学学报（自然科学版）,2012,36(6):90-94,5.基金项目
国家自然科学基金项目(31070515) （31070515）
国家自然科学青年基金项目(30900008) （30900008）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
