南京农业大学学报2012，Vol.35Issue(6)：30-34,5.
草莓镶脉病毒外壳蛋白的原核表达及多抗血清制备
Prokaryotic expression and antiserum preparation of coat protein of Strawberry vein banding virus
摘要
Abstract
The coat protein gene (cp) of Strawberry vein banding virus (SVBV ) was amplified by PCR. SVBV cp gene was ligated to the prokaryotic expression vector pET-32a,and the recombinant plasmid was transformed into Escherichia coli BL21 ( DE3 ). BL21 was induced by IPTG with a final concentration of 0.05 mmol·L-1 to express fusion protein. It was indicated that SVBV cp gene gained fusion expression by SDS-PAGE and Western-blot analysis. The recombinant fusion protein was purified with Ni2+-NTA resin,and the polyclonal antiserum was obtained by immunizing rabbit. Dot-ELISA detection method was established by utilizing the polyclonal antiserum. The detection result showed that the acquired specifically polyclonal antiserum could be used for quick detection of strawberry infected with SVBV.关键词
草莓镶脉病毒/外壳蛋白/原核表达/多抗血清Key words
Strawberry vein banding virus (SVBV) /coat protein/ prokaryotic expression/ polyclonal antiserum分类
农业科技引用本文复制引用
江彤,杨友志,丁菲,蒋磊,李祥宇,邓竹根,谢朝阳,宋培培..草莓镶脉病毒外壳蛋白的原核表达及多抗血清制备[J].南京农业大学学报,2012,35(6):30-34,5.基金项目
国家自然科学基金项目(30740033) （30740033）
安徽省科技厅自然科学基金项目(11040606M68) （11040606M68）
安徽省教育厅自然科学基金重点项目(KJ2011A120,KJ2012A113) （KJ2011A120,KJ2012A113）
