南京农业大学学报2012，Vol.35Issue(6)：104-110,7.
山羊DAZL基因的克隆及在山羊骨髓间充质干细胞中的表达
Cloning of goat DAZL gene and its expression in goat bone marrow mesenchymal stem cells
摘要
Abstract
DAZL(Deleted in Azoospermia-Like) ,a germ cell-specific gene,has shown to regulate meiosis during spermatogenesis and play important roles in germ cell development and differentiation. In this study,goat DAZL gene coding region 950 bp was successfully amplified by RT-PCR from goat testis,and the sequence analysis showed that the CDS region included 885 nucleotides(ORF),encoding for 295 amino acids,and containing typical RRM domain and DAZ repeat motif. The amino acid similarity of DAZL gene between goat and bovine was 97. 97%. The DAZL gene was then subcloned into eukaryotic express vector pEGFP-C1 to generate recombinant expression vector pEGFP-DAZL. Furthermore, we transfected pEGFP-DAZL plasmid into goat bone marrow mesenchymal stem cells (BMSC)by liposome,and its expression was identified by observation of fluorescence microscopy and RT-PCR.关键词
山羊/DAZL基因/序列分析/真核表达/骨髓间充质干细胞Key words
goat/DAZL gene/sequence analysis/eukaryotic expression/bone marrow mesenchymal stem cells(BMSC)分类
农业科技引用本文复制引用
张艳丽,钟部帅,樊懿萱,周峥嵘,王子玉,应诗家,郭蓉,王锋..山羊DAZL基因的克隆及在山羊骨髓间充质干细胞中的表达[J].南京农业大学学报,2012,35(6):104-110,7.基金项目
国家自然科学基金青年基金项目(31201802) （31201802）
转基因生物新品种培育重大专项(2011ZX08008-003) （2011ZX08008-003）
南京农业大学青年科技创新基金项目(KJ2010012) （KJ2010012）
