南京农业大学学报2012，Vol.35Issue(6)：131-136,6.
储藏温度和时间对转基因大豆外源基因及蛋白的影响
Effects of the storage temperature and time on cp4-epsps gene and protein in genetically modified soybean
摘要
Abstract
In this study, the effect of different storage temperatures (4,25 ,37 ℃ ) and times( 10,30,50,70,90 d)on exogenous cp4-epsps gene and protein of genetically modified soybean was investigated by PCR and Western-blot methods. The results suggested that there were no obvious changes in nucleic acid during the storage periods. However, the degradation product (relative molecular mass 46.2×103)of EPSPS protein was detected at 25 ℃ and 37 ℃. The conclusion revealed that the storage temperature and time can promote the degradation of exogenous EPSPS protein, but exert no effect on integrity of the exogenous cp4-epsps gene during the storage period(90 days).关键词
转基因大豆/cp4-epsps基因/储藏温度/储藏时间/PCR/Western-blotKey words
genetically modified soybean/cp4-epsps gene/storage temperature/storage time/PCR/Western-blot分类
轻工纺织引用本文复制引用
周兴虎,祝常青,吴洪洪,沈文飚,周光宏,黄明..储藏温度和时间对转基因大豆外源基因及蛋白的影响[J].南京农业大学学报,2012,35(6):131-136,6.基金项目
南京市生物农业项目(转基因农产品检测试剂盒研究与开发) （转基因农产品检测试剂盒研究与开发）
转基因生物新品种培育重大专项(2009ZX08012-014B) （2009ZX08012-014B）
