农业环境科学学报2012，Vol.31Issue(12)：2425-2432,8.
施肥对设施菜地氨氧化细菌群落和丰度的影响
Effects of Fertilization Treatments on Community Structure and Abundance of Ammonia-oxidizing Bacteria in Greenhouse Soil
摘要
Abstract
The effects of five fertilization treatments on the community structure and abundance of ammonia-oxidizing bacteria(AOB ) were determined by the combination of terminal restriction fragment length polymorphism(T-RFLP) and real-time quantitative PCR in the greenhouse soils in Wuwei City of Gansu Province. The results by cloning and sequencing of amoA gene fragments showed that the AOB community in soils for all treatments consisted of two major groups, i.e. Nitrosospira cluster 3 and Nitrosomanas cluster 7. The sequences related to Ni-trosospira cluster 3 were predominant. The highest abundance of the AOB both in 0～20 cm and 20～40 cm soil layers were detected in the fertilization treatment which is the half of the farmer's conventional amount( 1/2MNPK), while the amoA gene copy number was with 9.95×107 and 6.65×107 copies·g-1 soil respectively, which was 105.0% and 315.3% higher respectively in 0-20 cm and 20-40 cm soil layers for 1/2MNPK treatment than that in the unfertilized treatment. Both fertilizer types and soil layers were the important factors that deduced the changes of AOB community and abundance. The results had significant references to the further exploring characteristic and adaptation mechanism of AOB in the soil nitrogen cycle under facility cultivation conditions.关键词
氨氧化细菌/施肥/群落结构/T-RFLP/实时荧光定量PCRKey words
ammonia-oxidizing bacteria/ fertilization/ community structure/ terminal restriction fragment length polymorphism/ real-time分类
农业科技引用本文复制引用
王亚男,曾希柏,俄胜哲,白玲玉,李莲芳,苏世鸣,沈灵凤..施肥对设施菜地氨氧化细菌群落和丰度的影响[J].农业环境科学学报,2012,31(12):2425-2432,8.基金项目
"十二五"国家科技支撑计划2012BAD05B06 （）
公益性科研院所(中国农业科学院农业环境与可持续发展研究所)专项资金 （中国农业科学院农业环境与可持续发展研究所）
