农业环境科学学报2012，Vol.31Issue(12)：2500-2505,6.
饵料对蚯蚓粪细菌群落结构多样性的影响
Characterization of Bacterial Community Diversity in Various Vermicomposts Produced from Feedstocks
摘要
Abstract
The earthworms were fed with different feedstocks, such as cattle manure, pig manure and sludge. Then the bacterial community diversity between feedstock and vermicompost were determined by DGGE analysis of PCR-amplified bacterial 16S rDNA V3 region. The results showed that: (1 )The diversities of bacterial community in various vermicomposts were all decreased as compared with different feedstocks. The similarity coefficients of the bacterial community between vermicompost and feedstock were different among all the tested samples, which were from 15.3% to 37.7%;(2)Feeding with different kind of feedstocks resulted in the different similarity coefficient of bacteria] community among all the types of vermicomposts, but some vermicompost samples showed similar bacterial community diversity. The highest similarity value was found between two vermicomposts derived from cattle manure and pig manure, respectively, both of which were about one fold higher than their corresponding feedstocks;( 3 )The bacterial community diversity of fresh vermicomposts declined and tended to be stable with the extension of feeding time after changing the feedstock from pig manure to catde manure. The bacterial community diversity in feedstock could be changed after feeding by earthworm. The similarity coefficient between cattle manure and its corresponding vermicomposts was decreased from 33.2% on 0 d to 14.8% on 21 d, and then tended to be stable after 21 d. In sum, our data suggested that the bacterial community diversity in vermicomposts could be influenced by the types and conversion of feedstocks. This work will help our understanding of the mechanism for disposal of organic wastes using earthworm bioreactor.关键词
饵料/蚯蚓粪/PCR-DGGE/细菌群落多样性Key words
feedstock/ vermicompost/ PCR-DGGE/ bacterial community diversity分类
资源环境引用本文复制引用
陈浩,赵海涛,姚振飞,孙红军,胡健..饵料对蚯蚓粪细菌群落结构多样性的影响[J].农业环境科学学报,2012,31(12):2500-2505,6.基金项目
农业部十二五公益性农业行业科研专项(201103004) （201103004）
国家自然科学基金(31000939) （31000939）
江苏省农业科技支撑项目(BE2011361) （BE2011361）
