热带作物学报2012，Vol.33Issue(10)：1812-1818,7.DOI:10.3969/j.issn.1000-2561.2012.10.018
荔枝花蕊蛋白双向电泳体系的建立与优化
Establishment and Optimization of Two-dimensional Electrophoresis System for Proteins of Litchi Flower Buds
摘要
Abstract
The two-dimensional electrophoresis (2-DE) technology was used to demonstrate the protein profile of Litchi (Litchi chinensis Sonn., cultivar Yuanhong) flower buds. The key processes of sample extraction method, sample loading, gel concentrations, equilibrium time and staining methods of 2-DE system were optimized. Proteins were extracted by phenol extraction method. The results showed that more than 1 200 protein spots were detected by Image Master 2D Platinum with the parameters including pH 4-7 24 cm IPG strip, 1.2 mg protein sample loading, 12% T gel, 20 min equilibrium and Coomassie Brilliant Blue (CCB) R250 stain. The optimized system provided the foundation for further proteomics analysis of flower sex-differentiation in Litchi.关键词
荔枝/花蕊/蛋白质/双向电泳Key words
Litchi/ Flower bud/ Protein/ 2-DE分类
农业科技引用本文复制引用
黄榕辉,陈品品,王晓艳,盖永红,陈伟,王平..荔枝花蕊蛋白双向电泳体系的建立与优化[J].热带作物学报,2012,33(10):1812-1818,7.基金项目
福建省自然科学基金项目(No.2009J01064). （No.2009J01064）
