热带作物学报2012，Vol.33Issue(10)：1824-1828,5.DOI:10.3969/j.issn.1000-2561.2012.10.020
海南龙血树基于愈伤组织诱导途径的组培快繁技术体系优化
Optimization of Tissue Culture and Rapid Propagation Technical System Based on Callus Induction of Dracaena cambodiana
摘要
Abstract
Used the lateral and top buds of Dracaena cambodiana as the explants to induce callus tissue with that differentiated to seedling and induced dragon's blood at the same time. The results are as follows: The optimized culture medium for callus tissue induction and differentiation: 1. 1/2MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+ CM 50 ml/L+sugar 30 g/L+arrageenan 8.6 g/L, buds of induction were more and neat, induction rate was more than 90%; 2. The Optimized culture medium for subculture proliferation of buds: modified MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+CM 50 ml/L+sugar 30 g/L+arrageenan 8.6 g/L, proliferation coefficient was up to 5～6; 3. he Optimized culture medium for rooting: without strong seedling stage directly from seedling to rooting stages, 1/2MS+NAA 0.4 mg/L+GA 1.0 mg/L+sugar 30 g/L+arrageenan 8.6 g/L, rooting rate was up to 100%. (4) After dragon's blood induction, separation and extraction, using thin—layer chromatography and HPLC—control, the study found that 14 ingredients had higher similarity between induced and natural dragon's blood in HPLC peak shape. The callus tissue culture technique gave consideration to the production of seedling and dragon's blood, and provided new ideas for an efficient, fast, convenient scale production in the future.关键词
海南龙血树/愈伤组织诱导/血竭Key words
Dracaena cambodiana/ Callus tissue induction/ Dragon's blood分类
农业科技引用本文复制引用
罗冠勇,钟云芳,宋希强,戴好富,杨冬华..海南龙血树基于愈伤组织诱导途径的组培快繁技术体系优化[J].热带作物学报,2012,33(10):1824-1828,5.基金项目
林业公益性行业科研专项(No.201204604) （No.201204604）
海南省重点科技计划项目(No.ZDXM20120004) （No.ZDXM20120004）
农业公益性行业科研专项(No.201303117). （No.201303117）
