热带作物学报2012，Vol.33Issue(10)：1856-1860,5.DOI:10.3969/j.issn.1000-2561.2012.10.026
小菜蛾高致病力玫烟色棒束孢菌株的筛选
Screening of High Virulent Strain of Isaria fumosorosea Against Diamondback Moth, Plutella xylostella
摘要
Abstract
The diamondback moth (DBM), Plutella xylostella (L.) (Lepidoptera: Plutellidae), is one of the most destructive cosmopolitan insect pests of brassicaceous crops. It is the first crop insect reported to be resistant to DDT and now, in many crucifer producing regions, it shows significant resistance to almost every synthetic insecticide applied in the field. The present study examined the difference of growth rate, sporulation, germination rate, and extracellular protease production among eight Isaria fumosorosea strains isolated from Lepidoptera in China. Three strains RCEF218(K RCEF3423 and RCEF3496 with good biological characters were selected for further bioassay against the larvae of P. xylostellais. The strain RCEF3423 showed high virulence to P. xylostellais with a corrected mortality of 96.16%, a infection ratio of 91.67%, and a lethal time for 50% mortality (LT50) of 4.58 d at a concentration of 1 ×107 conidia/mL. The strain showed great potential for sustainable for use in microbial control of P. xylostella.关键词
小菜蛾/玫烟色棒束孢/生物学特性/生物测定/菌株筛选Key words
Plutella xylostella/ Lsaria fumosorosea/ Bbiological characteristics/ Bioassay/ Strain screening分类
农业科技引用本文复制引用
刘玉军,陈名君,汤强,王滨,黄勃..小菜蛾高致病力玫烟色棒束孢菌株的筛选[J].热带作物学报,2012,33(10):1856-1860,5.基金项目
农业公益性行业科研专项(No.201303029) （No.201303029）
林业公益性行业科研专项(No.201204506) （No.201204506）
环保行业科研专项(No.201009023) （No.201009023）
安徽省高校自然科学基金项目(No.KJ2012B218). （No.KJ2012B218）
