热带作物学报2012，Vol.33Issue(12)：2199-2205,7.DOI:10.3969/j.issn.1000-2561.2012.12.016
甘蔗核苷二磷酸激酶(NDPK1)基因克隆及表达分析
Molecular Cloning of Sugarcane NDPK1 Gene and Its Expression Analysis
摘要
Abstract
In this study, the full length of NDPK1 cDNA was obtained by RT-PCR and its expression was analyzed by quantitative real-time PCR. The results showed thatNDPKl was consisted of 686 bp with an open reading frame of 450 bp, encoding a polypeptide of 149 amino acids, and its GenBank accession number is JQ712580. Sequences alignment showed that the homology of cDNA sequence between sugarcane and sorghum bicolor NDPK1 gene was 96%, while the homology of amino acid between them was 98%. The deduced protein molecular weight was 16.83 Ku withPI 6.58 and hydrophobic from -2.089 to 2.033. The molecular structure prediction results suggested that the protein contained 44.3% α-helix, 24.83% loop, 20.13% extended strand and 60.3% β-meander. The results of quantitative real-time PCR analysis showed that the mRNA of NDPK1 was increased initially and then increased with time of PEC stress, and NDPK1 had the most abundant expression at 30 h of the treatment; Significant differences were observed between the treatments of PEG and PEG+Si at 18, 30, 40 h of the treatment.关键词
甘蔗/核苷二磷酸激酶/克隆/生物信息学分析/表达Key words
Sugarcane/ NDPK/ Clone/ Bioinformatics analysis/ Expression分类
农业科技引用本文复制引用
梁潘霞,李杨瑞,杨丽涛..甘蔗核苷二磷酸激酶(NDPK1)基因克隆及表达分析[J].热带作物学报,2012,33(12):2199-2205,7.基金项目
国家科技支撑计划项目(No.2007BAD30B00) （No.2007BAD30B00）
科技部国际合作项目(No.2008DFA30600、2009DFA30820) （No.2008DFA30600、2009DFA30820）
广西自然科学基金团队项目(No.2011GXNSFF018002) （No.2011GXNSFF018002）
广西科技攻关项目(桂科能No.0815011) （桂科能No.0815011）
广西农业科学院团队项目(桂农科No.2011YT01) （桂农科No.2011YT01）
广西农业科学院基本科研业务专项项目(No.201019). （No.201019）
