热带作物学报2012，Vol.33Issue(12)：2206-2213,8.DOI:10.3969/j.issn.1000-2561.2012.12.017
龙眼胚性愈伤组织NBS-LRR类抗性基因的同源克隆及序列分析
Cloning and Analysis of NBS-LRR Resistance Genes from Embryogenic Callus in Dimocarpus longan Lour.
摘要
Abstract
Longan(Dimocarpus longan Lour)resistance gene analog(RGA)was conducted using the longan embryogenic callus (EC)cDNA as material. 29 sequences in 51 positive clones were found homology to known R genes. Via sequences multiple alignment, the typical NBS motifs were found in these 29 sequences. 26 sequences contained intact NBS domain, including p-Loop/Kinase-la[GGV(I/M)GKTT], kinase-2[LVDDVW(D)], kinase-3a(GSRIIITTRD) and GLPL [GL (F)PL (F)AL], and 3 sequences seem like truncated NBS sequences and 2 of them contained stop condon. Phylogenetic analysis showed that 29 sequences could be devided into TNL group and non-TNL group, 2 sequences were devided into TNL group, and other 27 sequences were devided into 4 non-TNL groups, non-TNL-1 and non-TNL-2 subgroup showed homology to BED-NBS-LRR genes, non-TNL-1 subgroup kinase-2 [LLMDGLW]motif showed high difference to known R genes. Cloned non-TNL-1 member NBS39 full length cDNA by GeneRacer kit, NBS39 protein sequence N -end detected an obvious coiled -coil structure, pfam analysis detected NBS and LRR8 domains, but did not detected BED-finger structure.关键词
NBS/抗性基因/龙眼/胚性愈伤组织Key words
NBS/ Resistance gene/ Longan/ Embryogenic callus分类
农业科技引用本文复制引用
叶炜,林玉玲,赖钟雄..龙眼胚性愈伤组织NBS-LRR类抗性基因的同源克隆及序列分析[J].热带作物学报,2012,33(12):2206-2213,8.基金项目
国家自然科学基金(NO.31078717) （NO.31078717）
福建省重大农业科技平台建设项目(No.2008N2001). （No.2008N2001）
