四川大学学报（自然科学版）2012，Vol.49Issue(6)：1349-1356,8.DOI:10.3969/j.issn.0490-6756.2012.06.032
猪STAT4、STAT6基因克隆及组织表达研究
Signal transducer and activator of transcription 4 and 6 genes in pigs (Sus scrofa) : cDNA cloning and tissue expression
摘要
Abstract
The full-length cDNAs of STAT4 and STAT6 have been cloned from adult pig liver. STAT4 cDNA encodes a protein precursor of 748 amino acids, which shares high amino acid sequence identity with that of human (97%) , cattle (98%) , rat (96%) , mouse (96%). The full-length cDNA of STAT6 encodes a protein precursor of 847 amino acids which shares high amino acid sequence identity with that of human (93%), cattle (95%), rat (88%) and mouse (87%). Using RT-PCR assay, the mRNA expression of STAT4 and STAT6 genes were also examined in adult pig tissues. The results showed that they were widely expressed in nearly all of the tissues examined. In addition, the expression plasmids of pig STAT4 and STAT6 were also constructed in the present study. These data pave the way for further functional characterization of STAT4 and STAT6 genes in pigs.关键词
家猪/JAK-STAT/STAT4/STAT6/组织表达Key words
pig,JAK-STAT,STAT4 ,STAT6,tissue expression分类
生物科学引用本文复制引用
李芳莲,孟凤燕,王亚军,李娟..猪STAT4、STAT6基因克隆及组织表达研究[J].四川大学学报（自然科学版）,2012,49(6):1349-1356,8.基金项目
转基因生物新品种培育科技重大专项(2009zx08009-145B) （2009zx08009-145B）
