四川大学学报（自然科学版）2012，Vol.49Issue(6)：1375-1380,6.DOI:10.3969/j.issn.0490-6756.2012.06.036
播娘蒿叶绿体型△12脱饱和酶的功能研究
Further functional characterization of a chloroplast △12 fatty acid desaturases from Descurainia sophia
摘要
Abstract
A chloroplast A12 fatty acid desaturases gene from Descurainia Sophia (DsFAD2CHL) was isolated by RT-PCR and RACE. It has an open reading frame of 1344 bp, which encodes 447 amino acids. Expression of this cDNA in Saccharamyces cerevisiae revealed that the desaturase had a function that introduce an double bond into 18:1A9 to produce linoleic acid (18:2A9.12) , and the enzyme removing a plastidial signal peptide had a 3. 72//d higher desaturation conversion rate. Further phospholipids and acyl-CoAs pool analysis by GC/MS showed that the substrate of this enzyme was combined with phospholipids but not acyl-CoA.关键词
播娘蒿/脂肪酸脱饱和酶/底物类型/酰基辅酶A库Key words
Descurainia sophia, fatty acid desaturase, substrate types, acyl-CoA pool分类
生物科学引用本文复制引用
李湘,叶华勋,兰利琼,傅华龙,汪乐霓,陈波,卿人韦..播娘蒿叶绿体型△12脱饱和酶的功能研究[J].四川大学学报（自然科学版）,2012,49(6):1375-1380,6.基金项目
国家高技术发展计划(863计划)(2007AA09Z427,2008AA10Z409) （863计划）
教育部留学回国人员启动基金(20071108-18-8) （20071108-18-8）
国家自然科学基金面上项目(40976092) （40976092）
